Human CD14 Antibody

Catalog #: MAB3833
4 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
MAB3833-SP
MAB3833-500
MAB3833-100

All CD14 Antibodies

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD14 in sandwich ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 50040

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human CD14 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CD14 Biotinylated Antibody (Catalog # BAF383)

Standard: Recombinant Human CD14 Protein (Catalog # 383-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Alternate Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human CD14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
Authors: Komori M, Blake A, Greenwood M et al.
Ann Neurol
Immunologic biomarkers, morbidity and mortality among HIV patients hospitalised in a Tertiary Care Hospital in the Brazilian Amazon
Authors: WM Gama, CHM Frank, TVR Almeida, DS Dos Santos, YO Chaves, DF da Silva, PP Orlandi, FR Pereira, GF Magalhães, BJ Baptista, VL de Oliveir, AA da Silva B, MF Santana, RL Gonçalves, AG da Costa, MC Dos Santos, LC de Lima Fe, MVG Lacerda, PA Nogueira
BMC infectious diseases, 2021-08-26;21(1):876.
Species: Human
Sample Types: Serum
Applications: ELISA Capture
Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14.
Authors: Ma C, Chang W, Shi G, Chang B, Cheng S, Shih Y, Wu H
J Immunol, 2015-01-21;194(4):1905-15.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development