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Human Amphiregulin Antibody

Catalog #: MAB262
19 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB262-SP
MAB262-100
MAB262-500
Cell Proliferation Induced by Amphiregulin and Neutralization by Human Amphiregulin Antibody.
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Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Amphiregulin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Amphiregulin (AR) in Western blots. In this format, this antibody shows less than 5% cross-reactivity with recombinant human HB-EGF.
Source
Monoclonal Mouse IgG1 Clone # 31221
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Amphiregulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Amphiregulin (Catalog # 262-AR)
under non-reducing conditions only

Human Amphiregulin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Amphiregulin Biotinylated Antibody (Catalog # BAF262)

Standard: Recombinant Human Amphiregulin Protein (Catalog # 262-AR)

Neutralization
Measured by its ability to neutralize Amphiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 50 ng/mL Recombinant Human Amphiregulin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Amphiregulin and Neutralization by Human Amphiregulin Antibody. View Larger

Cell Proliferation Induced by Amphiregulin and Neutralization by Human Amphiregulin Antibody. Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND50 is typically 5-15 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiregulin

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5-10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Entrez Gene IDs
374 (Human); 11839 (Mouse)
Alternate Names
Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF

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Citations for Human Amphiregulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. RETRACTED: Blockade of TNF-alpha signaling suppresses the AREG-mediated IL-6 and IL-8 cytokines secretion induced by anti-Ro/SSA autoantibodies
    Authors: Margherita Sisto, Sabrina Lisi, Dario Domenico Lofrumento, Liana Cucci, Vincenzo Mitolo, Massimo D'Amore
    Laboratory Investigation
  2. Systems-Level Properties of EGFR-RAS-ERK Signaling Amplify Local Signals to Generate Dynamic Gene Expression Heterogeneity
    Authors: Alexander E. Davies, Michael Pargett, Stefan Siebert, Taryn E. Gillies, Yongin Choi, Savannah J. Tobin et al.
    Cell Systems
  3. Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA
    Authors: Makio Saeki, Hiroshi Egusa, Yuya Kamano, Yoshito Kakihara, Walid A. Houry, Hirofumi Yatani et al.
    PLoS ONE
  4. Interleukin-6–dependent epithelial fluidization initiates fibrotic lung remodeling
    Authors: Ian T. Stancil, Jacob E. Michalski, Corinne E. Hennessy, Kristina L. Hatakka, Ivana V. Yang, Jonathan S. Kurche et al.
    Science Translational Medicine
  5. CX3CL1 Promotes Breast Cancer via Transactivation of the EGF Pathway
    Authors: Manuel Tardáguila, Emilia Mira, Miguel A. García-Cabezas, Anna M. Feijoo, Miguel Quintela-Fandino, Iñigo Azcoitia et al.
    Cancer Research
  6. Tissue remodeling by an opportunistic pathogen triggers allergic inflammation
    Authors: Karen Agaronyan, Lokesh Sharma, Bharat Vaidyanathan, Keith Glenn, Shuang Yu, Charles Annicelli et al.
    Immunity
  7. TACE/ADAM-17 Phosphorylation by PKC-Epsilon Mediates Premalignant Changes in Tobacco Smoke-Exposed Lung Cells
    Authors: Hassan Lemjabbar-Alaoui, Sukhvinder S. Sidhu, Aklilu Mengistab, Marianne Gallup, Carol Basbaum
    PLoS ONE
  8. Sustained amphiregulin expression in intermediate alveolar stem cells drives progressive fibrosis
    Authors: Zhao, R;Wang, Z;Wang, G;Geng, J;Wu, H;Liu, X;Bin, E;Sui, J;Dai, H;Tang, N;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutations
    Authors: Miranda, AX;Kemp, J;Davidson, B;Bellomo, SE;Agan, V;Manoni, A;Marchiò, C;Croessmann, S;Park, BH;Hodges, E;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional
    Authors: IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz
    Nature Communications, 2021-07-27;12(1):4566.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. MAPK activity dynamics regulate non-cell autonomous effects of oncogene expression
    Authors: TJ Aikin, AF Peterson, MJ Pokrass, HR Clark, S Regot
    Elife, 2020-09-17;9(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis
    Authors: JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh
    Cell Rep, 2020-03-17;30(11):3806-3820.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis
    Authors: S Raimondo, L Saieva, E Vicario, M Pucci, D Toscani, M Manno, S Raccosta, N Giuliani, R Alessandro
    J Hematol Oncol, 2019-01-08;12(1):2.
    Species: Human
    Sample Types:
  14. CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin
    Authors: SJ Walker, LM Selfors, BL Margolis, JS Brugge
    PLoS ONE, 2018-11-15;13(11):e0207470.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.
    Authors: Stoll S, Stuart P, Lambert S, Gandarillas A, Rittie L, Johnston A, Elder J
    J Invest Dermatol, 2016-02-01;136(2):444-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  16. Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
    Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT
    J. Invest. Dermatol., 2010-04-29;130(8):2031-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  18. Regulation of Amphiregulin Gene Expression by beta -Catenin Signaling in Human Hepatocellular Carcinoma Cells: A Novel Crosstalk between FGF19 and the EGFR System
    Authors: Maria U. Latasa, Fabiana Salis, Raquel Urtasun, Oihane Garcia-Irigoyen, Maria Elizalde, Iker Uriarte et al.
    PLoS ONE
  19. Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls
    Authors: Donald M. Bryant, Konstantinos Sousounis, Duygu Payzin-Dogru, Sevara Bryant, Aaron Gabriel W. Sandoval, Jose Martinez Fernandez et al.
    npj Regenerative Medicine

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