Home / Carbonic Anhydrase IX/CA9 / Human Carbonic Anhydrase IX/CA9 Antibody

Human Carbonic Anhydrase IX/CA9 Antibody

Catalog #: MAB2188
8 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2188-SP
MAB2188-500
MAB2188-100
Detection of Carbonic Anhydrase IX/CA9 in U87-MG cells by Flow Cytometry.
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Citations (8)
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Human Carbonic Anhydrase IX/CA9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG2A Clone # 303123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
U‑87 MG human glioblastoma/astrocytoma cells
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase IX (Catalog # 2188-CA), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Carbonic Anhydrase IX Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Carbonic Anhydrase IX Biotinylated Antibody (Catalog # BAF2188)

Standard: Recombinant Human Carbonic Anhydrase IX Protein, CF (Catalog # 2188-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Carbonic Anhydrase IX/CA9 in U87-MG cells by Flow Cytometry. U87-MG cells were stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB2188, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

References
  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Citations for Human Carbonic Anhydrase IX/CA9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In vivo And Three-Dimensional Models over the Petri-dish
    Authors: R Alföldi, JÁ Balog, N Faragó, M Halmai, E Kotogány, P Neuperger, LI Nagy, LZ Fehér, GJ Szebeni, LG Puskás
    Cells, 2019-09-16;8(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion
    Authors: M Swayampaku, PC McDonald, M Vallejo, E Coyaud, SC Chafe, A Westerback, G Venkateswa, J Shankar, G Gao, EMN Laurent, Y Lou, KL Bennewith, CT Supuran, IR Nabi, B Raught, S Dedhar
    Oncogene, 2017-07-10;36(45):6244-6261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Global analysis of metastasis-associated gene expression in primary cultures from clinical specimens of clear-cell renal-cell carcinoma.
    Authors: Tan X, Zhai Y, Chang W, Hou J, He S, Lin L, Yu Y, Xu D, Xiao J, Ma L, Wang G, Cao T, Cao G
    Int. J. Cancer, 2008-09-01;123(5):1080-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Analysis of the Single-Cell Heterogeneity of Adenocarcinoma Cell Lines and the Investigation of Intratumor Heterogeneity Reveals the Expression of Transmembrane Protein 45A (TMEM45A) in Lung Adenocarcinoma Cancer Patients
    Authors: Patrícia Neuperger, József Á. Balog, László Tiszlavicz, József Furák, Nikolett Gémes, Edit Kotogány et al.
    Cancers (Basel)
  5. Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX
    Authors: Anne E.G. Lenferink, Paul C. McDonald, Christiane Cantin, Suzanne Grothé, Mylene Gosselin, Jason Baardsnes et al.
    mAbs
  6. Binding of the Phage Display Derived Peptide CaIX-P1 on Human Colorectal Carcinoma Cells Correlates with the Expression of Carbonic Anhydrase IX
    Authors: Vasileios Askoxylakis, Volker Ehemann, Shoaib Rana, Susanne Krämer, Nuh N. Rahbari, Jürgen Debus et al.
    International Journal of Molecular Sciences
  7. Cancer initiating-cells are enriched in the CA9 positive fraction of primary cervix cancer xenografts.
    Authors: Marie-Egyptienne DT, Chaudary N, Kalliomaki T et al.
    Oncotarget.
  8. Screening of a novel peptide targeting the proteoglycan-like region of human carbonic anhydrase IX
    Authors: Shoaib Rana, Felix Nissen, Thomas Lindner, Annette Altmann, Walter Mier, Juergen Debus et al.
    Mol Imaging

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