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Human IL-7 Antibody

Catalog #: MAB207
16 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB207-SP
MAB207-100
MAB207-500
Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody.
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Product Details
Citations (16)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-7 in Western blots. In ELISAs, does not cross-react with bovine FGF acidic, recombinant human (rh) G-CSF, rhGM-CSF, rhIL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, recombinant mouse IL-7, rhIL-8, porcine PDGF, rhTNF-alpha, rhTNF-beta, human TGF-beta 1, or porcine TGF-beta 1.
Source
Monoclonal Mouse IgG1 Clone # 7417
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-7
Accession # P13232
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑7 (Catalog # 207-IL)
under non-reducing conditions only

Human IL-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑7 Biotinylated Antibody (Catalog # BAF207)

Standard: Recombinant Human IL-7 Protein (Catalog # 207-IL)

Neutralization
Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND50) is typically 0.4-0.8 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. View Larger

Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207). The ND50 is typically 0.4-0.8 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. ­IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma  are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.

Long Name
Interleukin 7
Entrez Gene IDs
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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Citations for Human IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Ex vivo Co-culture of Lymphoid Tissue Stromal Cells and T Cells
    Authors: Ming Zeng, Ashley T. Haase
    Bio Protoc
  2. Potential implications of interleukin-7 in chronic wound healing
    Authors: ANNIE BARTLETT, ANDREW J. SANDERS, FIONA RUGE, KEITH G. HARDING, WEN G. JIANG
    Experimental and Therapeutic Medicine
  3. Macrophages are the primary effector cells in IL-7-induced arthritis
    Authors: SJ Kim, HJ Chang, MV Volin, S Umar, K Van Raemdo, A Chevalier, K Palasiewic, JW Christman, S Volkov, S Arami, M Maz, A Mehta, RK Zomorrodi, DA Fox, N Sweiss, S Shahrara
    Cell. Mol. Immunol., 2019-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  4. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions
    Authors: T Murakami, J Kim, Y Li, GE Green, A Shikanov, A Ono
    Nat Commun, 2018-06-22;9(1):2436.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016-09-02;6(0):32320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  6. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
    Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
    J Immunol, 2013-04-19;190(10):5256-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009-09-01;125(5):1092-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  10. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  11. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  12. Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys.
    Authors: Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP
    Blood, 2003-01-23;101(10):4209-18.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Whole Cells
  13. Modulation of peripheral T-cell function by interleukin-7 in rheumatoid arthritis
    Authors: Sarah M Churchman, Jehan J El-Jawhari, Agata N Burska, Rekha Parmar, Vincent Goëb, Philip G Conaghan et al.
    Arthritis Research & Therapy
  14. Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion
    Authors: Austin V. Stone, Kadie S. Vanderman, Jeffrey S. Willey, David L. Long, Thomas C. Register, Carol A. Shively et al.
    Osteoarthritis and Cartilage
  15. Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy
    Authors: Ming Zeng, Peter J. Southern, Cavan S. Reilly, Greg J. Beilman, Jeffrey G. Chipman, Timothy W. Schacker et al.
    PLoS Pathogens
  16. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity
    Authors: Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al.
    Frontiers in Immunology

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Reviews for Human IL-7 Antibody

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Human IL-7 Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human IL-7 Antibody MAB207
Human IL-7 Antibody
By Anonymous on 11/04/2017
Application: Flow Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster