Human Leptin/OB Biotinylated Antibody Summary
Val22-Cys167
Accession # P41159
Applications
Human Leptin/OB Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin/OB
Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.
A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.
- Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
- Tartaglia, L.A. et al. (1995) Cell 83:1263.
- Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Product Datasheets
Citations for Human Leptin/OB Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Dopamine Receptors in Human Adipocytes: Expression and Functions
Authors: Dana C. Borcherding, Eric R. Hugo, Gila Idelman, Anuradha De Silva, Nathan W. Richtand, Jean Loftus et al.
PLoS ONE
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Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice
Authors: Yuka Tomabechi, Takeshi Tsuruta, Shinichi Saito, Martin Wabitsch, Kei Sonoyama
Journal of Cell Communication and Signaling
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GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans
Authors: Satish Patel, Anna Alvarez-Guaita, Audrey Melvin, Debra Rimmington, Alessia Dattilo, Emily L. Miedzybrodzka et al.
Cell Metabolism
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Digital microfluidic assay for protein detection.
Authors: Mok J, Mindrinos M, Davis R, Javanmard M
Proc Natl Acad Sci U S A, 2014-01-21;111(6):2110-5.
Species: N/A
Sample Types: Recombinant Protein
Applications: Immunoassay Development -
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development, Functional Assay -
Leptin, blood pressure, and aerobic capacity in women.
Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG
Am. J. Hypertens., 2008-08-28;21(11):1245-50.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Authors: Emsley HC, Smith CJ, Gavin CM
BMC Neurol, 2007-02-28;7(0):5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64.
Species: Human
Sample Types: Follicular Fluid
Applications: ELISA Development -
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
Species: Human
Sample Types: Plasma
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