Feline IL-5 Biotinylated Antibody

Catalog #: BAM17951 Datasheet

Catalog #	Availability	Size / Price	Qty
BAM17951

All IL-5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Feline IL-5 Biotinylated Antibody Summary

Species Reactivity

Feline

Specificity

Detects feline IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant feline GM-CSF, recombinant equine, canine, mouse, human, or porcine IL-5 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 604308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Feline IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Feline IL‑5 Antibody (Catalog # MAB1795)

Standard: Recombinant Feline IL-5 Protein (Catalog # 1795-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin-5 (IL-5) is a 40 kDa secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the alpha -helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115 amino acid (aa) mature feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding alpha subunit with a signal transducing common beta subunit ( beta c) that is shared with receptor complexes for IL-3 and GM-CSF.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I