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Mouse CCL12/MCP-5 Biotinylated Antibody

Catalog #: BAF428
1 Citation Datasheet
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BAF428
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL12/MCP-5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL12/MCP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 0.07% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP-2, rhMCP-3, rhMCP-4, recombinant mouse (rm) MIP‑1 gamma, rmJE, and rmMARC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
Gly23-Gly104
Accession # Q62401
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)

Mouse CCL12/MCP-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL12/MCP‑5 Antibody (Catalog # MAB428)

Standard: Recombinant Mouse CCL12/MCP-5 Protein (Catalog # 428-P5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3 or CCR5.

References
  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Entrez Gene IDs
20293 (Mouse)
Alternate Names
C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12

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Citation for Mouse CCL12/MCP-5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcriptome analysis of the ependymal barrier during murine neurocysticercosis
    Authors: Pramod Kumar Mishra, Judy M Teale
    Journal of Neuroinflammation

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