Mouse CCL11/Eotaxin Biotinylated Antibody

Catalog #: BAF420
11 Citations 2 Reviews Datasheet

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BAF420

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All CCL11/Eotaxin Antibodies

Product Details

Citations (11)

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Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL11/Eotaxin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse CCL24/Eotaxin-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCL11/Eotaxin (R&D Systems, Catalog # 420-ME)
His24-Pro97
Accession # P48298

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)

Immunohistochemistry

5-15 µg/mL

See below

Mouse CCL11/Eotaxin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse CCL11/Eotaxin Antibody (Catalog # AF-420-NA)

Standard: Recombinant Mouse CCL11/Eotaxin Protein (Catalog # 420-ME)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

References

Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Alternate Names

C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse CCL11/Eotaxin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Helminth-induced CD19+CD23hi B cells modulate experimental allergic and autoimmune inflammation
Authors: Mark S. Wilson, Matthew D. Taylor, Mary T. O'Gorman, Adam Balic, Tom A. Barr, Kara Filbey et al.
European Journal of Immunology
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
J. Clin. Invest., 2007-10-01;117(10):2941-51.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006-08-01;177(3):1967-74.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
Authors: Reading PC, Symons JA, Smith GL
J. Immunol., 2003-02-01;170(3):1435-42.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development
P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
J. Leukoc. Biol., 2003-01-01;73(1):65-73.
Species: Mouse
Sample Types: Peritoneal Fluid
Applications: ELISA Development
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
Am. J. Pathol., 2002-04-01;160(4):1353-60.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development
Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors
Authors: JR Lee, I Appelmann, C Miething, TO Shultz, D Ruderman, D Kim, P Mallick, SW Lowe, SX Wang
Theranostics, 2018-02-03;8(5):1389-1398.

Ablation of Tumor Progression Locus 2 Promotes a Type 2 Th Cell Response in Ovalbumin-Immunized Mice
Authors: Wendy T. Watford, Chun-Chi Wang, Christos Tsatsanis, Lisa A. Mielke, Aristides G. Eliopoulos, Constantine Daskalakis et al.
The Journal of Immunology