Human IL-6R alpha Biotinylated Antibody Summary
Leu20-Asp358
Accession # P08887
Applications
Human IL-6R alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).
- Yamasaki et al. (1988) Science 241:825.
- Baumann et al. (1990) J. Biol. Chem. 265:19853.
- Hibi et al. (1990) Cell 63:1149.
- Schooltink et al. (1991) Eur. J. Biochem. 277:659.
- Novick et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa et al. (1990) J. Biochem. 108:673.
- Saito et al. (1991) J. Immunology 147:168.
Product Datasheets
Citations for Human IL-6R alpha Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
Authors: Komori M, Blake A, Greenwood M et al.
Ann Neurol
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Interleukin-6 controls recycling and degradation, but not internalization of its receptors
Authors: CM Flynn, B Kespohl, T Daunke, Y Garbers, S Düsterhöft, S Rose-John, J Haybaeck, J Lokau, S Aparicio-S, C Garbers
The Journal of Biological Chemistry, 2021-02-19;0(0):100434.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment
Authors: F Mimoto, K Tatsumi, S Shimizu, S Kadono, K Haraya, M Nagayasu, Y Suzuki, E Fujii, M Kamimura, A Hayasaka, H Kawauchi, K Ohara, M Matsushita, T Baba, H Susumu, T Sakashita, T Muraoka, K Aso, H Katada, E Tanaka, K Nakagawa, M Hasegawa, M Ayabe, T Yamamoto, S Tanba, T Ishiguro, T Kamikawa, T Nambu, T Kibayashi, Y Azuma, Y Tomii, A Kato, K Ozeki, N Murao, M Endo, J Kikuta, M Kamata-Sak, M Ishii, K Hattori, T Igawa
Cell Reports, 2020-12-22;33(12):108542.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture -
Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering.
Authors: Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K
J Immunol, 2015-08-28;195(7):3198-205.
Species: Human
Sample Types: Protein
Applications: ELISA Development -
A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor.
Authors: Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J
J Biol Chem, 2014-04-30;289(23):16336-48.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
Authors: Wood MW, Breitschwerdt EB, Gookin JL
Infect. Immun., 2010-11-29;79(2):708-15.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-Fr -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation
Authors: S Riethmuell, P Somasundar, JC Ehlers, CW Hung, CM Flynn, J Lokau, M Agthe, S Dsterh”ft, Y Zhu, J Gr”tzinger, I Lorenzen, T Koudelka, K Yamamoto, U Pickhinke, R Wichert, C Becker-Pau, M R„disch, A Albrecht, M Hessefort, D Stahnke, C Unverzagt, S Rose-John, A Tholey, C Garbers
PLoS Biol, 2017-01-06;15(1):e2000080.
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