Human Pentraxin 3/TSG-14 Biotinylated Antibody Summary
Glu18-Ser381
Accession # P26022
Applications
Human Pentraxin 3/TSG-14 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1‑3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).
Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23‑28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly up‑regulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG‑14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.
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- Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
- Lee, G.W. et al. (1993) J. Immunol. 150:1804.
- Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
- Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
- Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
- Introna, M. et al. (1996) Blood 87:1862.
- Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
- Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
- Rovere, P. et al. (2000) Blood 96:4300.
- Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
- Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.
Product Datasheets
Citations for Human Pentraxin 3/TSG-14 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
Authors: SA Aschenbroi, ER Lafontaine, MC Lopez, HV Baker, RJ Hogan
BMC Med Genomics, 2019-09-06;12(1):127.
Species: Human
Sample Types: Bacteria
Applications: Binding Assay -
FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation
Authors: ÁI Csincsi, Z Szabó, Z Bánlaki, B Uzonyi, M Cserhalmi, É Kárpáti, A Tortajada, JJE Caesar, Z Prohászka, TS Jokiranta, SM Lea, S Rodríguez, M Józsi
J. Immunol., 2017-05-22;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Functional Assay -
Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
Authors: Nobutaka Wakamiya
Biochim. Biophys. Acta, 2016-11-15;0(0):.
Applications: ICC -
Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation.
Authors: Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M
J Immunol, 2015-04-08;194(10):4963-73.
Species: Human
Sample Types: Protein
Applications: Binding Assay -
Atypical hemolytic uremic syndrome-associated variants and autoantibodies impair binding of factor h and factor h-related protein 1 to pentraxin 3.
J. Immunol., 2012-07-11;189(4):1858-67.
Species: Human
Sample Types: Protein
Applications: ELISA Development
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