Equine IL-10 Biotinylated Antibody

Catalog #: BAF1605 Datasheet

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BAF1605

All IL-10 Antibodies

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Equine IL-10 Biotinylated Antibody Summary

Species Reactivity

Equine

Specificity

Detects equine IL-10 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant canine IL-10 and recombinant porcine IL-10, and less than 0.4% cross-reactivity with recombinant human IL-10, recombinant mouse IL-10, recombinant rat IL-10, and recombinant feline IL-10 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant equine IL-10
Ser19-Asn178
Accession # Q28374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant equine IL-10 (Catalog # 1605-IL)

Equine IL-10 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Equine IL‑10 Antibody (Catalog # AF1605)

Standard: Recombinant Equine IL-10 Protein (Catalog # 1605-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper 2 (Th2) cells that inhibited the cytokine production by Th1 cells. It is a pleiotropic cytokine that regulates the immune and inflammatory responses of hematopoietic cells (1, 2). IL-10 has immunosuppressive activities and has been shown to inhibit the effector functions of monocyte/macrophage and CD4⁺T cells. Conversely, IL-10 has immunostimulatory activities and can induce the proliferation and cytotoxic activity of CD8⁺T cells and NK cells. IL-10 also regulates the growth and differentiation of B cells, mast cells, dendritic cells and neutrophils (1). The biological activities of IL-10 is mediated by the heteromeric IL-10 receptor complex, which is composed of the ligand-binding IL-10R alpha and the accessory IL-10R beta subunits. Both subunits belong to the class II cytokine receptor family. IL-10R beta is also utilized as a subunit in the heterodimer receptor complex for IL-22, IL-28 and IL-29. Besides IL-10, five novel cytokines (IL-19, -20, -22, -24, and -26) that share structural and limited sequence homology with IL-10 have been identified. These proteins constitute the IL-10 cytokine family (3).

Equine IL-10 cDNA encodes a 178 amino acid residue (aa) precursor protein with an 18 aa signal peptide and 160 aa mature protein that contains two potential N-linked glycosylation sites. Analogous to human IL-10, equine IL-10 likely exists as nondisulfide-linked homodimers. Equine IL-10 shares 71% and 78% aa sequence homology with mouse and human IL-10, respectively.

References

Moore, K. et al. (2001) Annu. Rev. Immunol. 19:683.
Mocellin, S. et al. (2003) Trends in Immunol. 23:36.
Conti, P. et al. (2003) Immunol. Letters 88:171.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF