Rat Lipocalin-2/NGAL Antibody Summary
Gln21-Asn198
Accession # P30152
Applications
Rat Lipocalin-2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin-2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Mouse Lipocalin-2/NGAL by Western Blot Visualization of expression of selected proteins by Western blot analysis. Protein extracts were prepared from livers of WT and Lcn2-/- mice that were subjected to indicated diets for 4 or 6 weeks (n=3 animals/group). Antibodies used are mentioned in the Section 2. GAPDH was used as the loading control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28725667), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).
- Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
- Devireddy, L.R. et al. (2001) Science 293:829.
- Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
- Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
- Flo, T.H. et al. (2004) Nature 432:917.
- Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
- Mori, K. et al. (2005) J. Clin. Invest. 115:610.
- Mishra, J. et al. (2005) Lancet 365:1231.
Product Datasheets
Citations for Rat Lipocalin-2/NGAL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma
Authors: Asimakopoulou A, Vucur M, Luedde T et al.
Cancers (Basel)
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Expression Analysis of Lipocalin 2 (LCN2) in Reproductive and Non-Reproductive Tissues of Esr1-Deficient Mice
Authors: Kessel, JC;Weiskirchen, R;Schröder, SK;
International journal of molecular sciences
Species: Transgenic Mouse
Sample Types: Serum, Tissue Homogenates, Whole Tissue
Applications: Western Blot, IHC -
Hepatocyte estrogen sulfotransferase inhibition protects female mice from Concanavalin A-induced T cell-mediated hepatitis independent of estrogens
Authors: J Wang, Z Zhang, J Guan, HC Tung, J Xie, H Huang, Y Chen, M Xu, S Ren, S Li, M Zhang, D Yang, W Xie
The Journal of Biological Chemistry, 2023-02-15;0(0):103026.
Species: Mouse, Transgenic Mouse
Sample Types: Protein, Whole Tissue
Applications: IHC, Western Blot -
Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology
Authors: C Poggel, T Adams, R Janzen, A Hofmann, O Hardt, E Roeb, SK Schröder, CG Tag, M Roderfeld, R Weiskirche
Biomedicines, 2022-09-06;10(9):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide
Authors: JY Lim, WW Jung, W Kim, KS Moon, D Sul
Scientific Reports, 2022-04-27;12(1):6837.
Species: Rat
Sample Types: Tissue Lysates
Applications: Western Blot -
Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria
Authors: P Klüber, SK Meurer, J Lambertz, R Schwarz, S Zechel-Gra, T Braunschwe, S Hurka, E Domann, R Weiskirche
International Journal of Molecular Sciences, 2021-12-05;22(23):.
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting
Authors: Erawan Borkham-Kamphorst, Eddy Van de Leur, Steffen K. Meurer, Eva M. Buhl, Ralf Weiskirchen
Frontiers in Pharmacology
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Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis
Authors: A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche
Data Brief, 2017-07-01;13(0):644-649.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Role of TGF-alpha in the progression of diabetic kidney disease
Authors: Josef G. Heuer, Shannon M. Harlan, Derek D. Yang, Dianna L. Jaqua, Jeffrey S. Boyles, Jonathan M. Wilson et al.
American Journal of Physiology-Renal Physiology
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Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity.
Authors: Chia W, Tan F, Ong W, Dawe G
Neurochem Int, 2015-05-21;87(0):43-59.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Lipocalin-2 (Lcn2) expression is mediated by maternal nutrition during the development of the fetal liver
Authors: William D. Rees, Susan M. Hay
Genes & Nutrition
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Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease
Authors: Sorina Georgiana Boaru, Erawan Borkham-Kamphorst, Lidia Tihaa, Ute Haas, Ralf Weiskirchen
Journal of Inflammation
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Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
Clin J Am Soc Nephrol, 2009-03-11;4(4):763-71.
Species: Rat
Sample Types: Urine
Applications: ELISA Development -
Lipocalin-2 in Fructose-Induced Fatty Liver Disease
Authors: J Lambertz, T Berger, TW Mak, J van Helden, R Weiskirche
Front Physiol, 2017-11-28;8(0):964.
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