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Human Neprilysin/CD10 Antibody

Catalog #: AF1182
8 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1182
AF1182-SP
Neprilysin/CD10 in Human Kidney.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neprilysin/CD10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neprilysin/CD10 in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Tyr52-Trp750
Accession # P08473
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
CD19+ human peripheral blood lymphocytes
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Neprilysin/CD10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1182)

Standard: Recombinant Human Neprilysin (CHO-expressed) Protein, CF (Catalog # 1182-ZNC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Neprilysin/CD10 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Neprilysin/CD10 in Human Kidney. Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human and Mouse Neprilysin/CD10 antibody by Western Blot. View Larger

Detection of Human and Mouse Neprilysin/CD10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, Recombinant Mouse Neprilysin (1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta  peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).

References
  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
  5. Itwata, N. et al. (2001) Science 292:1550.
Entrez Gene IDs
4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)
Alternate Names
Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase

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Citations for Human Neprilysin/CD10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. MME(+) fibro-adipogenic progenitors are the dominant adipogenic population during fatty infiltration in human skeletal muscle
    Authors: Fitzgerald G, Turiel G, Gorski T et al.
    Communications Biology
  2. Reduced Expression of Voltage-Gated Sodium Channel Beta 2 Restores Neuronal Injury and Improves Cognitive Dysfunction Induced by A beta 1-42
    Authors: Shan Li, Guo-Ji Yan, Ya-Xin Tan, Lu-Lu Xue, Ting-Hua Wang, Hao-Ran Zhao et al.
    Neural Plasticity
  3. Seminal-Plasma-Mediated Effects on Sperm Performance in Humans
    Authors: T Turunen, M Magris, M Malinen, J Kekäläinen
    Cells, 2022-07-08;11(14):.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Western Blot
  4. Neuroprotection induced by Navbeta2?knockdown in APP/PS1 transgenic neurons is associated with NEP regulation
    Authors: T Hu, SS Li, MN Lu, L Zhang, B Chen, R Mao, R Mei, YX Tan, S Li, YB Xiyang
    Mol Med Rep, 2019-06-20;20(2):2002-2011.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Activity Assay
  5. Vitamin D and Its Analogues Decrease Amyloid-? (A?) Formation and Increase A?-Degradation
    Authors: MOW Grimm, A Thiel, AA Lauer, J Winkler, J Lehmann, L Regner, C Nelke, D Janitschke, C Benoist, O Streidenbe, H Stötzel, K Endres, C Herr, C Beisswenge, HS Grimm, R Bals, F Lammert, T Hartmann
    Int J Mol Sci, 2017-12-19;18(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Activity Assay
  6. APP intracellular domain derived from amyloidogenic β- and γ-secretase cleavage regulates neprilysin expression
    Authors: Marcus O. W. Grimm, Janine Mett, Christoph P. Stahlmann, Sven Grösgen, Viola J. Haupenthal, Tamara Blümel et al.
    Frontiers in Aging Neuroscience
  7. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes.
    Authors: Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T
    J. Immunol., 2011-05-06;186(12):6925-32.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ELISA Development, ICC
  8. Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.
    Authors: Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M, Schmitt AL, Verleysdonk S, Kabisch D, Koeppen K, Siegel G, Proksch B, Kluba T, Eckert A, Kohle C, Schoneberg T, Northoff H, Schwab M, Gleiter CH
    Cell Death Differ., 2009-07-17;16(12):1599-614.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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