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Rat IL-1 beta /IL-1F2 Antibody

Catalog #: AF-501-NA
67 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF-501-NA
AF-501-SP
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody.
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Rat IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)
Val117-Ser268
Accession # Q63264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)
Immunocytochemistry
5-15 µg/mL
See below

Rat IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF501)

Standard: Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog # 501-RL)

Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL.

Immunocytochemistry IL‑1 beta /IL‑1F2 antibody in Rat Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Porcine IL-1 beta/IL-1F2 by Flow Cytometry View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Flow Cytometry Correlation of CD206 and Il-1 beta immunolabelling within the CD11b+ macrophage population following LD.A: Representative flow cytometry plots examine CD206+ and Il1b+ cell counts within the CD11b population following light damage. For the most part, Il-1 beta and CD206 cells occupied mutually distinct subsets within the population CD11b cells. B: Quantification of Il-1 beta +/CD206- and CD206+/Il-1 beta - cells as percentage of the CD11b+ population following LD. There was a sharp increase in the proportion of Il-1 beta +/CD206- cells immediately following 24hrs LD (P<0.05), though this then decreased dramatically afterward and was similar to control samples by 7 days (P>0.05). For CD206+/Il-1 beta - cells, there was no change in their proportion at 24hrs LD (P>0.05). At 3 days post-exposure however the proportion of CD206+/Il-1 beta - cells had tripled (P<0.05), though this was then reduced to near control proportions by 7 days post-exposure (P<0.05). The trend of both Il-1 beta +/CD206- and CD206+/Il-1 beta - cells across the time course were significant by ANOVA (P < 0.05); N = 5 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Porcine IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Western Blot The effect of chronic clomipramine treatment on the hippocampal NLRP3 inflammasome level in the CMS-treated rats.The protein levels of A IL-1 beta, B cleaved caspase-1, C NLRP3, D ASC, and E pro-caspase-1 (n = 4/group) were analyzed by western blot. All data are expressed as the mean ± SD. ##p < 0.01, ###p < 0.001 compared to saline-treated rats. *p < 0.05, **p <0.01, ***p < 0.001 compared to non-stressed control rats. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35688836), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Porcine IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Sustained IL-1 beta up-regulation in hippocampal astrocytes.IL-1 beta immunoreactivity was measured in GFAP-positive cells after LPS exposure or saline. Levels of IL-1 beta were found up-regulated from day 1 up to day 30. Higher magnification insets highlight the co-localization of IL-1 beta with GFAP (A). The ratio of IL-1 beta positive astrocytes in total astrocytes (GFAP positive cells) was quantified (B). Pictures show DG area, data are expressed as mean ± standard error of the mean (n = 4) and compared by 1-way analysis of variance followed with Boferroni post hoc analysis, **p<0.01, ***p<0.001 vs Control. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0106331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Rat IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Rat IL-1 beta/IL-1F2 by Immunocytochemistry/ Immunofluorescence Sustained IL-1 beta up-regulation in hippocampal astrocytes.IL-1 beta immunoreactivity was measured in GFAP-positive cells after LPS exposure or saline. Levels of IL-1 beta were found up-regulated from day 1 up to day 30. Higher magnification insets highlight the co-localization of IL-1 beta with GFAP (A). The ratio of IL-1 beta positive astrocytes in total astrocytes (GFAP positive cells) was quantified (B). Pictures show DG area, data are expressed as mean ± standard error of the mean (n = 4) and compared by 1-way analysis of variance followed with Boferroni post hoc analysis, **p<0.01, ***p<0.001 vs Control. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0106331), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Rat IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

67 Citations: Showing 1 - 10
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  1. Enhanced Activation of the S1PR2-IL-1?-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats
    Authors: Sancho-Alonso, M;Arenas, YM;Izquierdo-Altarejos, P;Martinez-Garcia, M;Llansola, M;Felipo, V;
    International journal of molecular sciences
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc
    Authors: YW Koo, CS Lim, A Darai, J Lee, W Kim, I Han, GH Kim
    Biomaterials research, 2023-03-29;27(1):26.
  3. An Injectable Engineered Cartilage Gel Improves Intervertebral Disc Repair in a Rat Nucleotomy Model
    Authors: Bhujel B, Yang SS, Kim HR et al.
    International journal of molecular sciences
  4. Disulfiram Ophthalmic Solution Inhibited Macrophage Infiltration by Suppressing Macrophage Pseudopodia Formation in a Rat Corneal Alkali Burn Model
    Authors: T Ikebukuro, T Arima, M Kasamatsu, Y Nakano, Y Tobita, M Uchiyama, Y Terashima, E Toda, A Shimizu, H Takahashi
    International Journal of Molecular Sciences, 2023-01-01;24(1):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat
    Authors: A Nagayach, R Bhaskar, I Patro
    Journal of chemical neuroanatomy, 2022-09-08;0(0):102160.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Long‐term priming of hypothalamic microglia is associated with energy balance disturbances under diet‐induced obesity
    Authors: María del Mar Fernández‐Arjona, Ana León‐Rodríguez, Jesús M. Grondona, María D. López‐Ávalos
    Glia
  7. Intermittent Theta Burst Stimulation Ameliorates Cognitive Deficit and Attenuates Neuroinflammation via PI3K/Akt/mTOR Signaling Pathway in Alzheimer’s-Like Disease Model
    Authors: Andjela Stekic, Milica Zeljkovic, Marina Zaric Kontic, Katarina Mihajlovic, Marija Adzic, Ivana Stevanovic et al.
    Frontiers in Aging Neuroscience
  8. Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia
    Authors: T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström
    EBioMedicine, 2022-02-16;77(0):103882.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy
    Authors: M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang
    Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Neuronal ApoE4 stimulates C/EBPbeta activation, promoting Alzheimer's disease pathology in a mouse model
    Authors: ZH Wang, Y Xia, Z Wu, SS Kang, JC Zhang, P Liu, X Liu, W Song, V Huin, CM Dhaenens, SP Yu, XC Wang, K Ye
    Progress in neurobiology, 2021-12-24;209(0):102212.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration
    Authors: Dragi? M, Mitrovi? N, Ad�i? M et al.
    ASN Neuro
  12. The selective estrogen receptor modulator tamoxifen protects against subtle cognitive decline and early markers of injury 24�h after hippocampal silent infarct in male Sprague-Dawley rats
    Authors: CA Finney, A Shvetcov, RF Westbrook, MJ Morris, NM Jones
    Hormones and behavior, 2021-07-06;134(0):105016.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Downregulation of CD73/A(2A)R-Mediated Adenosine Signaling as a Potential Mechanism of Neuroprotective Effects of Theta-Burst Transcranial Magnetic Stimulation in Acute Experimental Autoimmune Encephalomyelitis
    Authors: Dragi? M, Zeljkovi? M, Stevanovi? I et al.
    Brain Sciences
  14. Microglia activated by microbial neuraminidase contributes to ependymal cell death
    Authors: María del Mar Fernández-Arjona, Ana León-Rodríguez, María Dolores López-Ávalos, Jesús M. Grondona
    Fluids and Barriers of the CNS
  15. ApoE4 activates C/EBP&beta/&delta-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease
    Authors: ZH Wang, Y Xia, P Liu, X Liu, L Edgington-, K Lei, SP Yu, XC Wang, K Ye
    Progress in neurobiology, 2021-03-11;0(0):102032.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  16. Elamipretide (SS-31) Improves Functional Connectivity in Hippocampus and Other Related Regions Following Prolonged Neuroinflammation Induced by Lipopolysaccharide in Aged Rats
    Authors: Yang Liu, Huiqun Fu, Yan Wu, Binbin Nie, Fangyan Liu, Tianlong Wang et al.
    Frontiers in Aging Neuroscience
  17. New Inhibitory Effects of Cilnidipine on Microglial P2X7 Receptors and IL-1 beta Release: An Involvement in its Alleviating Effect on Neuropathic Pain
    Authors: Tomohiro Yamashita, Sawako Kamikaseda, Aya Tanaka, Hidetoshi Tozaki-Saitoh, Jose M. M. Caaveiro, Kazuhide Inoue et al.
    Cells
  18. CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrest
    Authors: Z Chen, X Xie, N Jiang, J Li, L Shen, Y Zhang
    Cell Death & Disease, 2021-02-15;12(2):184.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. Acute stress induces the rapid and transient induction of caspase-1, gasdermin D and release of constitutive IL-1 beta protein in dorsal hippocampus
    Authors: Matthew G. Frank, Michael V. Baratta, Kaixin Zhang, Isabella P. Fallon, Mikayleigh A. Pearson, Guozhen Liu et al.
    Brain, Behavior, and Immunity
  20. Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model
    Authors: TB Rusbana, AZ Agista, WD Saputra, Y Ohsaki, K Watanabe, A Ardiansyah, S Budijanto, T Koseki, H Aso, M Komai, H Shirakawa
    Nutrients, 2020-08-12;12(8):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells
    Authors: Amanda Ferreira Neves, Felipe Hertzing Farias, Silviane Fernandes de Magalhães, Dionéia Araldi, Marco Pagliusi, Claudia Herrera Tambeli et al.
    Frontiers in Physiology
  22. Of Mice and Monkeys: Neuroprotective Efficacy of the p38 Inhibitor BIRB 796 Depends on Model Duration in Experimental Glaucoma
    Authors: WS Lambert, S Pasini, JW Collyer, CR Formichell, P Ghose, BJ Carlson, DJ Calkins
    Sci Rep, 2020-05-22;10(1):8535.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  23. Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors
    Authors: MDM Fernández-, JM Grondona, P Fernández-, MD López-Ával
    J Neuroinflammation, 2019-12-02;16(1):245.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Microglial Morphometric Parameters Correlate With the Expression Level of IL-1 beta, and Allow Identifying Different Activated Morphotypes
    Authors: María del Mar Fernández-Arjona, Jesús M. Grondona, Pedro Fernández-Llebrez, María D. López-Ávalos
    Frontiers in Cellular Neuroscience
  25. Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation
    Authors: M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki
    Cell Rep, 2019-09-10;28(11):2923-2938.e8.
    Species: Rat
    Sample Types: Tissue Culture Supernates
    Applications: Western Blot
  26. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer's Disease
    Authors: ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye
    Cell Rep, 2019-07-16;28(3):655-669.e5.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Blockade of microglial adenosine A 2A receptor suppresses elevated pressure‐induced inflammation, oxidative stress, and cell death in retinal cells
    Authors: Inês Dinis Aires, Raquel Boia, Ana Catarina Rodrigues‐Neves, Maria Helena Madeira, Carla Marques, António Francisco Ambrósio et al.
    Glia
  28. Andrographolide relieved pathological pain generated by spared nerve injury model in mice
    Authors: HC Wang, HS Tsay, HN Shih, YA Chen, KM Chang, DC Agrawal, S Huang, YL Lin, MJ Lee
    Pharm Biol, 2018-12-01;56(1):124-131.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  29. Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection
    Authors: HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu
    J Chin Med Assoc, 2018-08-20;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
  30. Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy
    Authors: CH Jo, S Kim, JS Park, GH Kim
    Kidney Blood Press. Res., 2018-06-15;43(3):987-999.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  31. Glial interleukin-1? upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats
    Authors: P Zhang, RY Bi, YH Gan
    J Neuroinflammation, 2018-04-20;15(1):117.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  32. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms
    Authors: L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo
    J Neuroinflammation, 2018-02-08;15(1):36.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Neuroinflammation in Response to Intracerebral Injections of Different HMGB1 Redox Isoforms
    Authors: Hannah Aucott, Johan Lundberg, Henna Salo, Lena Klevenvall, Peter Damberg, Lars Ottosson et al.
    Journal of Innate Immunity
  34. Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica
    Authors: CC Shih, HR Hwang, CI Chang, HM Su, PC Chen, HM Kuo, PJ Li, HD Wang, KH Tsui, YC Lin, SY Huang, ZH Wen
    Int J Mol Sci, 2017-11-17;18(11):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  35. Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
    Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
    Mol Neurodegener, 2017-04-24;12(1):31.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  36. Inhibition of phagocytosis and pyroptosis of macrophages promotes Bartonella invasion into the bloodstream through lymphatic circulation
    Authors: Congli Yuan
    J. Infect. Dis., 2017-01-15;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Pyrrolidine Dithiocarbamate Prevents Neuroinflammation and Cognitive Dysfunction after Endotoxemia in Rats
    Authors: Min Hui Kan, Ting Yang, Hui Qun Fu, Long Fan, Yan Wu, Niccolò Terrando et al.
    Frontiers in Aging Neuroscience
  38. Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia
    Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo
    J Neuroinflammation, 2016-04-18;13(1):83.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  39. Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
    Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M
    PLoS ONE, 2015-12-02;10(12):e0143952.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. Synthetic Oligodeoxynucleotides Containing Multiple Telemeric TTAGGG Motifs Suppress Inflammasome Activity in Macrophages Subjected to Oxygen and Glucose Deprivation and Reduce Ischemic Brain Injury in Stroke-Prone Spontaneously Hypertensive Rats
    Authors: Jing Zhao, Yongshan Mou, Joshua D. Bernstock, Dace Klimanis, Sixian Wang, Maria Spatz et al.
    PLOS ONE
  41. Tumor necrosis factor enhances the sleep-like state and electrical stimulation induces a wake-like state in co-cultures of neurons and glia
    Authors: Kathryn A. Jewett, Ping Taishi, Parijat Sengupta, Sandip Roy, Christopher J. Davis, James M. Krueger
    European Journal of Neuroscience
  42. Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
    Authors: Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A
    J Neuroinflammation, 2015-06-10;12(0):115.
    Species: Rat
    Sample Types: Tissue Culture Supernates
    Applications: Neutralization
  43. Neuroinflammation Induced by Intracerebroventricular Injection of Microbial Neuraminidase
    Authors: Pablo Granados-Durán, María D. López-Ávalos, Jesús M. Grondona, María del Carmen Gómez-Roldán, Manuel Cifuentes, Margarita Pérez-Martín et al.
    Frontiers in Medicine
  44. The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
    Authors: Silva G, Lopes P, Fonoff E, Pagano R
    J Neuroinflammation, 2015-01-20;12(0):10.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  45. Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal.
    Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D
    Neuroscience, 2014-10-19;284(0):283-9.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  46. Vinpocetine inhibits amyloid-beta induced activation of NF-kappa B, NLRP3 inflammasome and cytokine production in retinal pigment epithelial cells
    Authors: Ruozhou Tom Liu, Aikun Wang, Eleanor To, Jiangyuan Gao, Sijia Cao, Jing Z. Cui et al.
    Experimental Eye Research
  47. Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
    Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T
    PLoS ONE, 2014-08-29;9(8):e106331.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  48. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
    Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
    J Neurosci, 2014-02-05;34(6):2231-43.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  49. Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
    Authors: Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J
    Invest Ophthalmol Vis Sci, 2013-03-01;54(3):2225-37.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  50. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
    J Neuroinflammation, 2012-07-23;9(0):181.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  51. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
    Authors: Faustino JV, Wang X, Johnson CE
    J. Neurosci., 2011-09-07;31(36):12992-3001.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  52. Acute Cocaine Increases Interleukin-1 beta mRNA and Immunoreactive Cells in the Cortex and Nucleus Accumbens
    Authors: Barbara A. Cearley, James M. Blindheim, Barbara A. Churchill, Cassia N. Krueger, Kelly Churchill
    Neurochemical Research
  53. Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
    Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
    J. Neurochem., 2011-02-09;117(1):164-74.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  54. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2010-12-02;116(2):217-26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  55. Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
    Authors: Summy-Long JY, Hu S
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-09-16;297(5):R1532-45.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  56. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
    Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
    J. Clin. Invest., 2009-01-12;119(2):295-304.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  57. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008-06-09;76(8):3577-86.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development
  58. Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
    Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
    Endocrinology, 2008-01-03;149(4):1436-50.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  59. IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
    Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
    Biochem. Biophys. Res. Commun., 2007-11-08;365(2):375-80.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  60. Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
    Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2007-07-06;293(3):H1839-46.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  61. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006-09-29;181(1):57-67.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development
  62. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006-06-01;15(3):407-21.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  63. Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
    Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O
    Pediatr. Res., 2005-04-21;57(6):896-901.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  64. Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
    Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
    Neuroscience, 2005-01-01;132(3):625-31.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004-01-14;556(0):193-202.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  66. A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
    Authors: Guo L, LaDu MJ, Van Eldik LJ
    J. Mol. Neurosci., 2004-01-01;23(3):205-12.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  67. Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
    Authors: Nadeau S, Rivest S
    J. Neurosci., 2003-07-02;23(13):5536-44.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization

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Rat IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum Species: Rat
ELISA Rat IL-1 beta /IL-1F2 Antibody AF-501-NA