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Recombinant Human Mer Fc Chimera Protein, CF

Catalog #: 891-MR
5 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
891-MR-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
FAQs
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Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Mer Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) Mer Fc Chimera at 10 µg/mL (100 µL/well) can bind rhGas6 (Catalog # 885-GSB) with a linear range of 0.03-1 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Mer protein
Human Mer
(Arg26-Ala499)
Accession # AAB60430.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
AAB60430.1
N-terminal Sequence
Analysis
Arg26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
78 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

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891-MR

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

891-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Citations for Recombinant Human Mer Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
    Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
    Cell Death & Disease, 2021-05-25;12(6):538.
    Species: Human
    Sample Types: Protein
    Applications: SPR
  2. TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
    Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
    Neuron, 2018-03-07;97(5):1023-1031.e7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Immunoprecipitation
  3. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Targeting Axl with an high-affinity inhibitory aptamer.
    Authors: Cerchia, Laura, Esposito, Carla L, Camorani, Simona, Rienzo, Anna, Stasio, Loredana, Insabato, Luigi, Affuso, Andrea, de Franciscis, Vittorio
    Mol Ther, 2012-08-21;20(12):2291-303.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
    Authors: Uehara H, Shacter E
    J. Immunol., 2008-02-15;180(4):2522-30.
    Species: Human
    Sample Types: Phospholipid Vesicles
    Applications: Bioassay

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Reviews for Recombinant Human Mer Fc Chimera Protein, CF

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human Mer Fc Chimera Protein, CF
By Anonymous on 11/12/2020
Application: In vitro bioactivity in cell culture
Recombinant Human Mer Fc Chimera Protein, CF
By Anonymous on 04/03/2018
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human Mer Fc Chimera Protein, CF 891-MR