Recombinant Rat CXCL3/CINC-2 alpha Protein

Catalog #: 516-CA 1 Review Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
516-CA-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
516-CA-025

R&D Systems Recombinant Proteins and Enzymes

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All CXCL3/GRO gamma/CINC-2/DCIP-1 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CXCL3/CINC-2 alpha Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED₅₀ for this effect is 3-6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 4-20 ng/mL.

Source

E. coli-derived rat CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Arg33-Ser100

Accession #

Q10746

N-terminal Sequence
Analysis

Arg33

Predicted Molecular Mass

7.6 kDa

Product Datasheets

516-CA (with carrier)

Product Datasheet

516-CA/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

516-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

516-CA/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta and CINC-3 (also named MIP-2) constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 but not IL-8. CINC-2 alpha, CINC-2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue which also contain CINC-1 and CINC-3. Based on amino acid (aa) sequence analysis of the purified CINC-2 alpha protein and sequencing of the CINC-2 beta cDNA clone, both mature CINC-2 alpha and CINC-2 beta were shown to contain 68 aa residues. The amino acid sequences of the two CINC-2 proteins are identical except for three carboxy-terminal residues. CINC-2 beta cDNA encodes a 100 aa residue precursor protein with a 32 aa residue signal peptide that is removed to yield the mature secreted protein. At the protein sequence level, mature CINC-2 proteins are 63% identical to CINC-1 and 80% identical to CINC-3. CINC-2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or LPS-induced inflammatory exudate. Other cell types known to produce CINC-2 proteins include activated macrophages and fibroblasts.

Recombinant and natural CINC-2 proteins have been shown to be specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3-specific receptor as well as a second common receptor shared by all CINCs.

References

Nakagawa, H. et al. (1994) Biochem. J. 301:545.
Watanabe, K. et al. (1989) J. Biol. Chem. 264:19559.
Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
Shibata, F. et al. (1995) Eur. J. Biochem. 231:306.
Al-Mokdad, M. et al. (1996) Biol. Pharm. Bull. 19:879.
Murakami, K. et al. (1997) Biochem. and Biophys. Res. Commun. 232:562.
Watanabe, K. et al. (1991) Exp. Mol. Pathol. 55:30.
Nakagawa, H. et al. (1996) Biochem. and Biophys. Res. Commun. 220:945.

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3