Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein

Catalog #: 435-FA
5 Citations 1 Review Datasheet

Carrier Free

Catalog #	Availability	Size / Price	Qty
435-FA-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
435-FA-050

R&D Systems Recombinant Proteins and Enzymes

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All Fas/TNFRSF6/CD95 Proteins and Enzymes

Product Details

Citations (5)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.03‑0.1 µg/mL in the presence of 20 ng/mL recombinant human Fas-ligand.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Fas/TNFRSF6/CD95 protein

Mouse FAS (Gly14 - Arg169) Accession # P25446	DIEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P25446

N-terminal Sequence
Analysis

Gly14

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

55 kDa, reducing conditions

Product Datasheets

435-FA (with carrier)

Product Datasheet

435-FA/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

435-FA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

435-FA/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO‑1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1 ‑ 3). Mouse Fas cDNA encodes 327 amino acids (aa) that include a 21 aa signal peptide, a 148 aa extracellular domain (ECD) that contains three cysteine‑ich TNFR repeats, a 17 aa transmembrane sequence, and a 141 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature mouse Fas ECD shares 68% aa sequence identity with rat Fas, and 53 ‑ 55% with human, feline, bovine and porcine Fas. One potentially secreted 63 aa isoform diverges at aa 61 (5). The Fas ligand (FasL, TNFSF6) is a type II transmembrane protein of the TNF family that can be expressed on activated T‑lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2). Polymorphisms of FasL impart differing activity of Fas depending on mouse strain (6). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death‑Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus‑transformed lymphocytes and tumor cells. It is an essential mediator in the activation‑induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune‑privileged tissues, infiltrating Fas‑bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1 ‑ 3). The Fas pathway also appears to cross‑communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References

Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
Strasser, A. et al. (2009) Immunity 30:180.
Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
SwissProt accession P25446.
NCBI accession NP_001140180.
Kayagaki, N. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3914.
Thorburn, A. (2003) Cellular Signaling 16:139.
Barreiro, R. et al. (2004) J. Immunol. 173:1519.
Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.

Long Name

Fibroblast-associated

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Alternate Names

Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Related Research Areas

Citations for Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies
Authors: M Kamiya, F Mizoguchi, K Kawahata, D Wang, M Nishibori, J Day, C Louis, IP Wicks, H Kohsaka, S Yasuda
Nature Communications, 2022-01-10;13(1):166.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Signaling by the Epstein-Barr virus LMP1 protein induces potent cytotoxic CD4+ and CD8+ T cell responses
Authors: IK Choi, Z Wang, Q Ke, M Hong, Y Qian, X Zhao, Y Liu, HJ Kim, J Ritz, H Cantor, K Rajewsky, KW Wucherpfen, B Zhang
Proc. Natl. Acad. Sci. U.S.A., 2018-01-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Deficiency in the mitochondrial apoptotic pathway reveals the toxic potential of autophagy under ER stress conditions.
Authors: Deegan S, Saveljeva S, Logue S, Pakos-Zebrucka K, Gupta S, Vandenabeele P, Bertrand M, Samali A
Autophagy, 2014-01-01;10(11):1921-36.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia.
Authors: Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA
Cell Death Differ., 2007-10-26;15(1):143-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
J. Immunol., 2006-10-15;177(8):5041-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay