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Recombinant Human IGF-I R/IGF1R Protein, CF

Catalog #: 391-GR
9 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
391-GR-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGF-I R/IGF1R Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1).
Source
Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932
Accession #
P08069
N-terminal Sequence
Analysis
Glu31 and Asp741
Predicted Molecular Mass
102.9 kDa (single chain); 81.0 kDa ( alpha chain) & 21.9 kDa ( beta chain)
SDS-PAGE
160 kDa, 125 kDa, 50 kDa & 40 kDa, reducing conditions

Product Datasheets

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391-GR

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

391-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References
  1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

Citations for Recombinant Human IGF-I R/IGF1R Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Sequence tolerance of immunoglobulin variable domain framework regions to non-canonical intradomain disulfide linkages
    Authors: Kim, DY;Kandalaft, H;Lowden, MJ;Yang, Q;Rossotti, MA;Robotham, A;Kelly, JF;Hussack, G;Schrag, JD;Henry, KA;Tanha, J;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  2. Brain Delivery of IGF1R5, a Single-Domain Antibody Targeting Insulin-like Growth Factor-1 Receptor
    Authors: A Yogi, G Hussack, H van Faasse, AS Haqqani, CE Delaney, E Brunette, JK Sandhu, M Hewitt, T Sulea, K Kemmerich, DB Stanimirov
    Pharmaceutics, 2022-07-12;14(7):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: SPR
  3. Defining the epitope of a blood-brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor
    Authors: J Sheff, P Wang, P Xu, M Arbour, L Masson, H van Faasse, G Hussack, K Kemmerich, E Brunette, D Stanimirov, JJ Hill, J Kelly, F Ni
    Scientific Reports, 2021-02-19;11(1):4284.
    Species: Human
    Sample Types: Whole Cells
    Applications: Surface Plasmon Resonance
  4. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer
    Authors: NO Yunn, M Park, S Park, J Lee, J Noh, E Shin, SH Ryu
    Nucleic Acids Research, 2021-01-25;49(2):700-712.
    Species: Human
    Sample Types: DNA
    Applications: Bioassay
  5. Development and preclinical evaluation of cixutumumab drug conjugates in a model of insulin growth factor receptor I (IGF-1R) positive cancer
    Authors: VR Solomon, E Alizadeh, W Bernhard, A Makhlouf, SV Hartimath, W Hill, A El-Sayed, K Barreto, CR Geyer, H Fonge
    Sci Rep, 2020-10-29;10(1):18549.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  6. Efficacy of the Antibody-Drug Conjugate W0101 in Preclinical Models of IGF-1 Receptor Overexpressing Solid Tumors
    Authors: B Akla, M Broussas, N Loukili, A Robert, C Beau-Larvo, M Malissard, N Boute, T Champion, JF Haeuw, A Beck, M Perez, C Dreyfus, M Pavlyuk, E Chetaille, N Corvaia
    Mol. Cancer Ther., 2019-10-08;0(0):.
    Species: Human, Mouse
    Sample Types: In Vivo, Recombinant Protein
    Applications: ELISA, In Vivo
  7. Direct integrin binding to insulin-like growth factor-2 through the C-domain is required for insulin-like growth factor receptor type 1 (IGF1R) signaling
    Authors: DM Cedano Pri, Y Cheng, CC Chang, J Yu, YK Takada, Y Takada
    PLoS ONE, 2017-09-05;12(9):e0184285.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA
  8. A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies
    PLoS ONE, 2016-09-15;11(9):e0163113.
    Applications: Surface Plasmon Resonance
  9. Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
    Authors: Back K, Arnqvist HJ
    Growth Horm. IGF Res., 2008-08-09;19(2):101-11.
    Applications: ELISA (Standard)

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Recombinant Human IGF-I R/IGF1R Protein, CF
By Anonymous on 08/01/2017
Application: Apoptosis assay