Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein
Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein Summary
Product Specifications
Ser2-Lys246
Analysis
Product Datasheets
377-HB (with carrier)
377-HB/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
377-HB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
377-HB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) stimulates cell proliferation of the MCF-7 human breast cancer cell line in a serum free assay. The ED50 for this effect is 2.5-12.5 ng/mL.
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1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Background: Neuregulin-1 beta 1/NRG1 beta 1
The Neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
- Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
- Adlkofer, K. and C. Lai (2000) Glia 29:104.
- Garratt, A.N. et al., (2000) BioEssays 22:987.
Citations for Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination
Authors: G Della-Flor, ER Wilson, E Hurley, B He, BW O'Malley, Y Poitelon, L Wrabetz, ML Feltri
Elife, 2021-09-14;10(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers
Authors: T Osada, ZC Hartman, J Wei, G Lei, AC Hobeika, WR Gwin, MA Diniz, N Spector, TM Clay, W Chen, MA Morse, HK Lyerly
Breast Cancer Res., 2018-08-09;20(1):90.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis
Authors: G Scapin, VP Dandey, Z Zhang, W Prosise, A Hruza, T Kelly, T Mayhood, C Strickland, CS Potter, B Carragher
Nature, 2018-02-28;0(0):.
Applications: Bioassay -
Uridine-5'-Triphosphate Partially Blocks Differentiation Signals and Favors a more Repair State in Cultured rat Schwann Cells
Authors: M Palomo-Gue, JM Cosgaya, A Gella, N Casals, C Grijota-Ma
Neuroscience, 2018-01-11;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Identification of Anti-EGFR and Anti-ErbB3 Dual Variable Domains Immunoglobulin (DVD-Ig) Proteins with Unique Activities.
Authors: Gu J, Yang J, Chang Q, Liu Z, Ghayur T, Gu J
PLoS ONE, 2015-05-21;10(5):e0124135.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
Stem Cells Transl Med, 2014-01-17;3(3):334-45.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cellular Effects of HER3-Specific Affibody Molecules.
Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
PLoS ONE, 2012-06-29;7(6):e40023.
Species: Human
Sample Types: Buffer
Applications: Binding Assay -
Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice.
Authors: Casazza A, Finisguerra V, Capparuccia L
J. Clin. Invest., 2010-07-26;120(8):2684-98.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells.
Authors: Sheng, Qing, Liu, Xinggang, Fleming, Eleanor, Yuan, Karen, Piao, Huiying, Chen, Jinyun, Moustafa, Zeinab, Thomas, Roman K, Greulich, Heidi, Schinzel, Anna, Zaghlul, Sara, Batt, David, Ettenberg, Seth, Meyerson, Matthew, Schoeberl, Birgit, Kung, Andrew L, Hahn, William, Drapkin, Ronny, Livingston, David M, Liu, Joyce F
Cancer Cell, 2010-03-16;17(3):298-310.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration.
Authors: Yamauchi J, Miyamoto Y, Chan JR, Tanoue A
J. Cell Biol., 2008-04-21;181(2):351-65.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006-07-01;10(1):39-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Authors: Barkho BZ, Song H, Aimone JB
Stem Cells Dev., 2006-06-01;15(3):407-21.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Neuregulin-1-beta1 enters brain and spinal cord by receptor-mediated transport.
Authors: Kastin AJ, Akerstrom V, Pan W
J. Neurochem., 2004-02-01;88(4):965-70.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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