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Recombinant Human M-CSF R/CD115 Fc Chimera Protein

Catalog #: 329-MR
6 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
329-MR-100/CF

With Carrier

Catalog # Availability Size / Price Qty
329-MR-100
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Product Details
Citations (6)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human M-CSF R/CD115 Fc Chimera Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. (1989) Biotechnology 7:710. The ED50 for this effect is 0.004-0.012 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF (Catalog # 216-MC).
Source
Mouse myeloma cell line, NS0-derived human M-CSF R/CD115 protein
Human M-CSF R
(Ile20-Glu512)
Accession # CAA27300		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
CAA27300
N-terminal Sequence
Analysis
Ile20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
81 kDa (monomer)
SDS-PAGE
109-128 kDa, reducing conditions

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329-MR (with carrier)

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329-MR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

329-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

329-MR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased
M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References
  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
Long Name
Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs
1436 (Human); 12978 (Mouse)
Alternate Names
CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms

Citations for Recombinant Human M-CSF R/CD115 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. TLR signalling in human antigen-presenting cells regulates MR1-dependent activation of MAIT cells
    Authors: JE Ussher, B van Wilgen, RF Hannaway, K Ruustal, P Phalora, A Kurioka, TH Hansen, CB Willberg, RE Phillips, P Klenerman
    Eur J Immunol, 2016-05-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells
    PLoS ONE, 2016-05-20;11(5):e0155791.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Enhanced antagonism of BST-2 by a neurovirulent SIV envelope
    J Clin Invest, 2016-05-09;0(0):.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms
    Sci Rep, 2016-05-06;6(0):25481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Exocytosis of Macrophage Lysosomes Leads to Digestion of Apoptotic Adipocytes and Foam Cell Formation
    Authors: AS Haka, VC Barbosa-Lo, HJ Lee, DJ Falcone, CA Hudis, AJ Dannenberg, FR Maxfield
    J Lipid Res, 2016-04-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
    Authors: Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S
    Nat. Med., 2010-05-02;16(5):571-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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