Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein
Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein Summary
Product Specifications
| Human Fas (Arg17-Asn173) Accession # Q5T9P3 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
326-FS (with carrier)
326-FS/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
326-FS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
326-FS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Fas/TNFRSF6/CD95
Fas, also known as APO‑1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1-3). The 335 amino acid (aa) human Fas includes a 25 aa signal peptide, a 148 aa extracellular domain (ECD) with three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 145 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature human Fas ECD shares 55%, 58%, 62%, 63%, and 64% aa sequence identity with mouse, rat, feline, bovine and porcine Fas, respectively. A human Fas isoform of 314 aa that lacks the transmembrane sequence is secreted by resting lymphocytes, while isoforms of 149, 132, 103 and 86 aa that also lack the DD and show substitutions for parts of the TNFR repeats are less prominently expressed (4-6). All appear to block the extrinsic apoptosis pathway induced by the Fas ligand (FasL, TNFSF6), a type II transmembrane protein of the TNF family that can be expressed on activated T‑lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2, 6). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death‑Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation‑induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune‑privileged tissues, infiltrating Fas‑bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1-3). The Fas pathway also appears to cross‑communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).
- Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
- Strasser, A. et al. (2009) Immunity 30:180.
- Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
- SwissProt accession P25445.
- Liu, C. et al. (1995) Biochem. J. 310:957.
- Papoff, G. et al. (1996) J. Immunol. 156:4622.
- Thorburn, A. (2003) Cellular Signaling 16:139.
- Barreiro, R. et al. (2004) J. Immunol. 173:1519.
- Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.
Citations for Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
Scientific Reports, 2022-02-25;12(1):3234.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
Int J Mol Sci, 2020-04-21;21(8):.
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture -
Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli
Authors: AE Dodi, IO Ajayi, C Chang, M Beard, SL Ashley, SK Huang, VJ Thannickal, DJ Tschumperl, TH Sisson, JC Horowitz
Respir. Res., 2018-05-10;19(1):91.
Applications: ELISA (Standard) -
Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression.
Authors: Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH
Mol. Cell. Biol., 2006-04-01;26(8):3071-84.
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IFN-beta induces caspase-mediated apoptosis by disrupting mitochondria in human advanced stage colon cancer cell lines.
Authors: Juang SH, Wei SJ, Hung YM, Hsu CY, Yang DM, Liu KJ, Chen WS, Yang WK
J. Interferon Cytokine Res., 2004-04-01;24(4):231-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional and phenotypic changes in circulating lymphocytes from hospitalized zambian children with measles.
Authors: Ryon JJ, Moss WJ, Monze M, Griffin DE
Clin. Diagn. Lab. Immunol., 2002-09-01;9(5):994-1003.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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