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Recombinant Mouse Pentraxin 3/TSG-14 Protein

Catalog #: 2166-TS
6 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
2166-TS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2166-TS-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Pentraxin 3/TSG-14 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhC1q at 5 µg/mL (100 µL/well) can bind rmPentraxin 3 with a linear range of 0.25-15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Pentraxin 3/TSG-14 protein
Glu18-Ser381, with a C-terminal 6-His tag
Accession #
P48759
N-terminal Sequence
Analysis
Glu18
Structure / Form
Multimer consisting of as many as ten non-covalently and covalently linked subunits
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
46 kDa, reducing conditions

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2166-TS (with carrier)

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2166-TS/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2166-TS

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2166-TS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pentraxin 3/TSG-14

Pentraxin 3, also known as TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1 - 3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).

Mature secreted PTX3 contains a pentaxin-like domain at its carboxy-terminus that shares 23 - 28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belong to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similar to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly upregulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG-14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). An in vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild-type during inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

References
  1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  2. Breviario, F. et al. (1992) JBC. 267:22190.
  3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
  4. Osmand, A.P. et al. (1977) PNAS, USA 74:739.
  5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Rev. 7:191.
  6. Bottazzi, B. et al. (1997) JBC. 272:32817.
  7. Introna, M. et al. (1996) Blood, 87:1862.
  8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
  9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
  10. Rovere, P. et al. (2000) Blood 96:4300.
  11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs
5806 (Human); 19288 (Mouse)
Alternate Names
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Citations for Recombinant Mouse Pentraxin 3/TSG-14 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Inactivation of pentraxin 3 suppresses M2-like macrophage activity and immunosuppression in colon cancer
    Authors: Chen, FW;Wu, YL;Cheng, CC;Hsiao, YW;Chi, JY;Hung, LY;Chang, CP;Lai, MD;Wang, JM;
    Journal of biomedical science
    Species: Mouse
    Sample Types: Lung Perfusate
    Applications: In Vivo
  2. Menin directs regionalized decidual transformation through epigenetically setting PTX3 to balance FGF and BMP signaling
    Authors: M Liu, W Deng, L Tang, M Liu, H Bao, C Guo, C Zhang, J Lu, H Wang, Z Lu, S Kong
    Nature Communications, 2022-02-22;13(1):1006.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The Long Pentraxin 3 (PTX3) Suppresses Immunity to Cutaneous Leishmaniasis by Regulating CD4+ T Helper Cell Response
    Authors: G Gupta, Z Mou, P Jia, R Sharma, R Zayats, SM Viana, L Shan, A Barral, VS Boaventura, TT Murooka, A Soussi-Gou, CI de Oliveir, JE Uzonna
    Cell Reports, 2020-12-15;33(11):108513.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Pentraxin 3 contributes to neurogenesis after traumatic brain injury in mice
    Authors: C Zhou, H Chen, JF Zheng, ZD Guo, ZJ Huang, Y Wu, JJ Zhong, XC Sun, CJ Cheng
    Neural Regen Res, 2020-12-01;15(12):2318-2326.
    Species: Mouse
    Sample Types: In Vivo, Spheroid
    Applications: Bioassay, In Vivo
  5. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015-02-19;11(2):e1004649.
    Species: Mouse
    Sample Types: Virus
    Applications: Bioassay
  6. Pentraxin 3 mediates neurogenesis and angiogenesis after cerebral ischaemia.
    Authors: Rodriguez-Grande B, Varghese L, Molina-Holgado F, Rajkovic O, Garlanda C, Denes A, Pinteaux E
    J Neuroinflammation, 2015-01-24;12(0):15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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