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Recombinant Mouse NOV/CCN3 Protein, CF

Catalog #: 1976-NV
5 Citations Datasheet
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1976-NV-050
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Citations (5)
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Recombinant Mouse NOV/CCN3 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. rmNOV, immobilized at 10 µg/mL, 100 µL/well, will mediate >25% Balbc/3T3 cell adhesion (3 x 104 cells/well added).
Source
Mouse myeloma cell line, NS0-derived mouse NOV/CCN3 protein
Ser26-Ile354, with a C-terminal 10-His tag
Accession #
Q64299
N-terminal Sequence
Analysis
Ser26
Predicted Molecular Mass
37.4 kDa
SDS-PAGE
54 kDa, reducing conditions

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1976-NV

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1976-NV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1 - 4). NOV/CCN3 contains an N-terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 354 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and canine NOV/CCN3, and 78% aa identity with bovine NOV/CCN3. NOV/CCN3 also shows 38 - 50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1 - 4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C-terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and  alpha 5 beta 1 mediates endothelial cell adhesion, induces chemotaxis and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

References
  1. Perbal, B. (2004) Lancet 363:62.
  2. Perbal, B. (2006) Cell Commun. Signal. 4:3.
  3. Martinerie, C. et al. (1994) Oncogene 9:2729.
  4. Snaith, M. R. et al. (1996) Genomics 38:425.
  5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
  6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
  7. Fu, C. T. et al. (2004) J. Biol. Chem. 279:36943.
  8. Li, C. L. et al. (2002) Mol. Pathol. 55:250.
  9. Planque, N. et al. (2006) J. Cell. Biochem. Apr. 5 [e-pub ahead of print].
  10. Lin, C. G. et al. (2003) J. Biol. Chem. 278:24200.
  11. Lin, C. G. et al. (2003) J. Biol. Chem. 280:8229.
  12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.
Long Name
Nephroblastoma Overexpressed Gene
Entrez Gene IDs
4856 (Human); 18133 (Mouse)
Alternate Names
CCN family member 3; CCN3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; protein NOV homolog

Citations for Recombinant Mouse NOV/CCN3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway
    Authors: Luan, Y;Zhang, H;Ma, K;Liu, Y;Lu, H;Chen, X;Liu, Y;Zhang, Z;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
    Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
    Cell Stem Cell, 2020-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Regulatory T cells promote myelin regeneration in the central nervous system
    Authors: Y Dombrowski, T O'Hagan, M Dittmer, R Penalva, SR Mayoral, P Bankhead, S Fleville, G Eleftheria, C Zhao, M Naughton, R Hassan, J Moffat, J Falconer, A Boyd, P Hamilton, IV Allen, A Kissenpfen, PN Moynagh, E Evergren, B Perbal, AC Williams, RJ Ingram, JR Chan, RJ Franklin, DC Fitzgerald
    Nat. Neurosci, 2017-03-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  4. Cancer stem cells regulate cancer-associated fibroblasts via activation of Hedgehog signaling in mammary gland tumors
    Authors: G Valenti, HM Quinn, GJ Heynen, L Lan, JD Holland, R Vogel, A Wulf-Golde, W Birchmeier
    Cancer Res, 2017-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CCN3 (NOV) is a negative regulator of CCN2 (CTGF) and a novel endogenous inhibitor of the fibrotic pathway in an in vitro model of renal disease.
    Authors: Riser BL, Najmabadi F, Perbal B, Peterson DR, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC
    Am. J. Pathol., 2009-04-09;174(5):1725-34.
    Applications: ELISA (Standard)

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