Zebrafish VEGF Antibody

Catalog #: AF1247 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF1247
AF1247-SP

Cell Proliferation Induced by VEGF165and Neutralization by Zebrafish VEGF Antibody.

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Zebrafish VEGF Antibody Summary

Species Reactivity

Zebrafish

Specificity

Detects zebrafish VEGF in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant human VEGF₁₆₅, recombinant mouse VEGF₁₆₄, and recombinant rat VEGF₁₆₄ (under non‑reducing conditions) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant zebrafish VEGF₁₆₅
Ala24-Arg188
Accession # O73682

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Zebrafish VEGF₁₆₅ (Catalog # 1247-ZV)

Neutralization

Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.3-0.6 µg/mL in the presence of 80 ng/mL Recombinant Zebrafish VEGF₁₆₅.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by VEGF₁₆₅and Neutralization by Zebrafish VEGF Antibody. Recombinant Zebrafish VEGF₁₆₅(Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Zebrafish VEGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1247). The ND₅₀ is typically 0.3-0.6 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF₁₆₅ shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF₁₆₅, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish (FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as up‑regulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).

References

Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
Laio, W. et al. (1997) Development 124:381.
Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
Thurston, G. (2002) J. Anat. 200:575.
Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor