Viral HIV-1 Gag p24 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9539-100
MAB9539-SP
Detection of HIV‑1 Gag p24 by Western Blot.
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Product Details
Citations (1)
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Viral HIV-1 Gag p24 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral HIV-1 Gag p24 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2230C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant viral HIV-1 Gag p24 
Pro149-Gly354
Accession # Q77YG1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of HIV-1 Gag p24 antibody by Western Blot. View Larger

Detection of HIV‑1 Gag p24 by Western Blot. Western blot shows recombinant HIV Gag p24. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB9539) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIV-1 Gag p24 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs
155030 (Viral)
Alternate Names
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)

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Citation for Viral HIV-1 Gag p24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
    Authors: Haldrup, J;Andersen, S;Labial, ARL;Wolff, JH;Frandsen, FP;Skov, TW;Rovsing, AB;Nielsen, I;Jakobsen, TS;Askou, AL;Thomsen, MK;Corydon, TJ;Thomsen, EA;Mikkelsen, JG;
    Nucleic acids research
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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