Viral HCMV IL-10 Biotinylated Antibody

Catalog #: BAF117
9 Citations Datasheet

Catalog #	Availability	Size / Price	Qty
BAF117

All IL-10 Antibodies

Product Details

Citations (9)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral HCMV IL-10 Biotinylated Antibody Summary

Species Reactivity

Viral

Specificity

Detects Viral HCMV IL-10 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant Viral HCMV IL‑10 (R&D Systems, Catalog # 117-VL)
Ala26-Lys176
Accession # AAF63437

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Viral HCMV IL‑10 (Catalog # 117-VL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

References

Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
Spencer, J.V. et al. (2002) J. Virol. 76:1285.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Viral HCMV IL-10 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Human Cytomegalovirus pUL11, a CD45 Ligand, Disrupts CD4 T Cell Control of Viral Spread in Epithelial Cells
Authors: SA Osanyinlus, J Zischke, R Jacobs, EM Weissinger, TF Schulz, PC Kay-Fedoro
MBio, 2022-11-29;0(0):e0294622.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children
Authors: Joseph L. Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung et al.
Haematologica
Challenging the Conventional Interpretation of HCMV Seronegativity
Authors: S Waters, S Lee, A Irish, P Price
Microorganisms, 2021-11-18;9(11):.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection
Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
Sci Rep, 2017-01-05;7(0):39710.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
BMC Biotechnol, 2013-10-05;13(0):82.
Species: Virus
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages
Authors: Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa
Clinical Cancer Research
Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
Clin. Cancer Res., 2011-04-13;17(14):4642-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
Authors: Chang WL, Baumgarth N, Yu D, Barry PA
J. Virol., 2004-08-01;78(16):8720-31.
Species: Virus
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Development, Western Blot