Ubiquitin K48 Linkage Antibody
Ubiquitin K48 Linkage Antibody Summary
Accession # P0CG47
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with MG132. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Ubiquitin K48 Linkage Monoclonal Antibody (Catalog # A-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ubiquitin at approximately 75-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Ubiquitin by Western Blot. 25 ng of each linkage of recombinant diubiquitin was run on a 10-20% SDS-PAGE gel prior to blotting on PVDF membrane. Western blots were developed using anti-K48 mAb (A-101, upper panel) or anti-ubiquitin mAb (MAB701, lower panel) primaries at 0.5 µg/ml. The appropriate HRP-labeled anti-rabbit or anti-mouse (R&D Systems HAF008 or HAF007) secondary antibodies were used at a 1:2000 dilution. A single band of appropriate size was detected only in the K48-linked diubiquitin lane using A-101.
Preparation and Storage
Background: Ubiquitin
Polyubiquitin chains are composed of ubiquitin monomers that are covalently linked through isopeptide bonds (other than linear, or “Met1-linked” polyubiquitin). Isopeptides are formed between a lysine residue of one Ubiquitin molecule and the Cterminal glycine residue of another Ubiquitin molecule. Seven of the seventy-six amino acids in ubiquitin are lysine residues that can participate in polyubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes. K48-linked polyubiquitin chains attached to substrate proteins often serve as a recognition sequence for targeting and destruction of the substrate by the 26S Proteasome. This antibody detects the K48 linkage. It does not detect monoubiquitin or ubiquitin liked via any other lysine residue. Reactivity across all species is anticipated.
Product Datasheets
Product Specific Notices
Not for use in humansCitations for Ubiquitin K48 Linkage Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Protein thiol oxidation in the rat lung following e-cigarette exposure
Authors: J Wang, T Zhang, CJ Johnston, SY Kim, MJ Gaffrey, D Chalupa, G Feng, WJ Qian, MD McGraw, C Ansong
Redox Biol, 2020-10-10;37(0):101758.
Species: Rat
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Validation of Babesia proteasome as a drug target
Authors: M Jalovecka, D Hartmann, Y Miyamoto, L Eckmann, O Hajdusek, AJ O'Donoghue, D Sojka
Int J Parasitol Drugs Drug Resist, 2018-08-07;8(3):394-402.
Applications: Western Blot
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