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TGF-beta 1 Biotinylated Antibody

Catalog #: BAF240
41 Citations 4 Reviews Datasheet
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BAF240
Product Details
Citations (41)
FAQs
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Reviews (4)
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TGF-beta 1 Biotinylated Antibody Summary

Specificity
Detects human TGF-beta 1 in ELISAs and Western blots. In sandwich immunoassays, when used in combination with the rhTGF‑ beta  sRII/Fc chimera (Catalog # 341-BR), approximately 15% cross-reactivity with recombinant human (rh) Latent TGF‑ beta 1 is observed, 1% cross-reactivity with rhTGF-beta 1.2 is observed, and 3% cross-reactivity with recombinant amphibian (ra) TGF-beta 5 is observed. When used in combination with the monoclonal capture antibody (Catalog # MAB2402), approximately 5% cross‑reactivity with TGF-beta 1.2 is observed and 1% cross-reactivity with rhTGF-beta 2, recombinant chicken TGF-beta 3, and raTGF-beta 5 is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta 1 (Catalog # 240-B)

Human TGF-beta 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Recombinant Human TGF-beta RII Fc Chimera Protein, CF (Catalog # 341-BR)

Capture Reagent: TGF-beta 1, 2, 3 Antibody (Catalog # MAB1835)

Capture Reagent: TGF‑ beta 1 Antibody (Catalog # MAB2402)

Standard: Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1

TGF- beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity (1). TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

 

References
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 1
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

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Citations for TGF-beta 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Heligmosomoides polygyrus Infection Can Inhibit Colitis through Direct Interaction with Innate Immunity
    Authors: Long Hang, Tommy Setiawan, Arthur M. Blum, Joseph Urban, Korynn Stoyanoff, Seiji Arihiro et al.
    The Journal of Immunology
  2. Impact of Dietary Lavender Essential Oil on the Growth and Fatty Acid Profile of Breast Muscles, Antioxidant Activity, and Inflammatory Responses in Broiler Chickens
    Authors: Shimaa A. Amer, Ahmed A. A. Abdel-Wareth, Ahmed Gouda, Gehan K. Saleh, Arwa H. Nassar, Wafaa R. I. A. Sherief et al.
    Antioxidants (Basel)
  3. Heligmosomoides polygyrus bakeri Induces Tolerogenic Dendritic Cells that Block Colitis and Prevent Antigen-Specific Gut T Cell Responses
    Authors: Arthur M. Blum, Long Hang, Tommy Setiawan, Joseph P. Urban, Korynn M. Stoyanoff, John Leung et al.
    The Journal of Immunology
  4. Severity of the TGN1412 trial disaster cytokine storm correlated with IL-2 release
    Authors: David Eastwood, Chris Bird, Paula Dilger, Jason Hockley, Lucy Findlay, Stephen Poole et al.
    British Journal of Clinical Pharmacology
  5. A Curative Immune Profile One Week after Treatment of Indian Kala-Azar Patients Predicts Success with a Short-Course Liposomal Amphotericin B Therapy
    Authors: Smriti Mondal, Pradyot Bhattacharya, Mehebubar Rahaman, Nahid Ali, Rama Prosad Goswami
    PLoS Neglected Tropical Diseases
  6. T Cell Surveillance of Oncogene-Induced Prostate Cancer Is Impeded by T Cell-Derived TGF-beta 1 Cytokine
    Authors: Moses K. Donkor, Abira Sarkar, Peter A. Savage, Ruth A. Franklin, Linda K. Johnson, Achim A. Jungbluth et al.
    Immunity
  7. On the Role IL-4/IL-13 Heteroreceptor Plays in Regulation of Type 1 Diabetes
    Authors: TK Ukah, AN Cattin-Roy, W Chen, MM Miller, S Barik, H Zaghouani
    J. Immunol., 2017-06-23;0(0):.
  8. The Tyrphostin Agent AG490 Prevents and Reverses Type 1 Diabetes in NOD Mice
    Authors: Abdoreza Davoodi-Semiromi, Clive H. Wasserfall, Chang Qing Xia, Rhonda M. Cooper-DeHoff, Martin Wabitsch, Michael Clare-Salzler et al.
    PLoS ONE
  9. Stromal regulation of vessel stability by MMP14 and TGFbeta
    Authors: Nor E. Sounni, Kerstin Dehne, Leon van Kempen, Mikala Egeblad, Nesrine I. Affara, Ileana Cuevas et al.
    Disease Models & Mechanisms
  10. Murine CD4 T Cells Produce a New Form of TGF-beta as Measured by a Newly Developed TGF-beta Bioassay
    Authors: Takatoku Oida, Howard L. Weiner
    PLoS ONE
  11. Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
    Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
    Anticancer Res., 2018-04-01;38(4):2007-2014.
  12. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation
    Authors: Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al.
    Biology of Blood and Marrow Transplantation
  13. FoxP3+RORgammat+ T helper intermediates display suppressive function against autoimmune diabetes
    Authors: Danielle M. Tartar, Amie M. VanMorlan, Xiaoxiao Wan, F. Betul Guloglu, Renu Jain, Cara L. Haymaker et al.
    The Journal of Immunology
  14. Autocrine Transforming Growth Factor-beta 1 Promotes In Vivo Th17 Cell Differentiation
    Authors: Ilona Gutcher, Moses K. Donkor, Qian Ma, Alexander Y. Rudensky, Richard A. Flavell, Ming O. Li
    Immunity
  15. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  16. Targeting caveolae to pump bispecific antibody to TGF-beta into diseased lungs enables ultra-low dose therapeutic efficacy
    Authors: AH Kadam, K Kandasamy, T Buss, B Cederstrom, C Yang, S Narayanapi, J Rodriguez, MD Levin, J Koziol, B Olenyuk, Z Borok, A Chrastina, JE Schnitzer
    PLoS ONE, 2022-11-22;17(11):e0276462.
    Species: Rat
    Sample Types: In Vivo, Tissue Homogenates
    Applications: Neutralization, Western Blot
  17. Cancer immunotherapy via targeted TGF-&beta signalling blockade in TH cells
    Authors: S Li, M Liu, MH Do, C Chou, EG Stamatiade, BG Nixon, W Shi, X Zhang, P Li, S Gao, KJ Capistrano, H Xu, NV Cheung, MO Li
    Nature, 2020-10-21;0(0):.
    Species: Mouse
    Sample Types: Reference Standard
  18. Circulating Picomolar Levels of CCL2 Downregulate Ongoing Chronic Experimental Autoimmune Encephalomyelitis by Induction of Regulatory Mechanisms
    Authors: N Kaushansky, E Bakos, S Becker-Her, I Shachar, A Ben-Nun
    J. Immunol., 2019-09-04;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  19. TGF??1 upregulates the expression of lncRNA?ATB to promote atherosclerosis
    Authors: H Yu, S Ma, L Sun, J Gao, C Zhao
    Mol Med Rep, 2019-03-29;19(5):4222-4228.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  20. Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGF? enhance the efficacy of cancer immunotherapy
    Authors: R Ravi, KA Noonan, V Pham, R Bedi, A Zhavoronko, IV Ozerov, E Makarev, A V Artemov, PT Wysocki, R Mehra, S Nimmagadda, L Marchionni, D Sidransky, IM Borrello, E Izumchenko, A Bedi
    Nat Commun, 2018-02-21;9(1):741.
    Applications: ELISA Development (Detection)
  21. Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease.
    Authors: Kaushansky N, Eisenstein M, Boura-Halfon S, Hansen B, Nielsen C, Milo R, Zeilig G, Lassmann H, Altmann D, Ben-Nun A
    J Biol Chem, 2015-04-24;290(24):15260-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  22. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
    Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
    Nat Commun, 2015-02-19;6(0):6353.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1).
    Authors: Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G
    J Biol Chem, 2014-12-29;290(7):4422-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  24. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
    Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
    J Immunol, 2013-12-11;192(2):804-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  26. Activation of Toll-like receptor 3 augments myofibroblast differentiation.
    Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
    Am. J. Respir. Cell Mol. Biol., 2008-11-06;40(6):654-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  27. In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
    Authors: Saurer L, McCullough KC, Summerfield A
    J. Immunol., 2007-09-15;179(6):3504-14.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  28. Antigen-induced regulatory T cells in HBV chronically infected patients.
    Authors: Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L
    Virology, 2007-07-23;368(1):41-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  30. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    Immunol. Lett., 2006-03-03;105(2):159-66.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  31. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  32. Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3.
    Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M
    J Exp Med, 2003-12-15;198(12):1875-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  34. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000-05-15;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  35. Inhibition of renal fibrosis with a human CXCL9‐derived glycosaminoglycan‐binding peptide
    Authors: Fariba Poosti, Mohammad Ayodhia Soebadi, Helena Crijns, Alexandra De Zutter, Mieke Metzemaekers, Nele Berghmans et al.
    Clinical & Translational Immunology
  36. Activation of latent transforming growth factor-beta 1, a conserved function for pregnancy-specific beta 1-glycoproteins
    Authors: James Warren, Michelle Im, Angela Ballesteros, Cam Ha, Tom Moore, Fanny Lambert et al.
    MHR: Basic science of reproductive medicine
  37. Muscleblind-like 1 is required for normal heart valve development in vivo
    Authors: Ryan J. Coram, Samantha J. Stillwagon, Anuradha Guggilam, Michael W. Jenkins, Maurice S. Swanson, Andrea N. Ladd
    BMC Developmental Biology
  38. DQB1*0602 rather than DRB1*1501 confers susceptibility to multiple sclerosis-like disease induced by proteolipid protein (PLP)
    Authors: Nathali Kaushansky, Daniel M Altmann, Chella S David, Hans Lassmann, Avraham Ben-Nun
    Journal of Neuroinflammation
  39. ‘Multi-Epitope-Targeted’ Immune-Specific Therapy for a Multiple Sclerosis-Like Disease via Engineered Multi-Epitope Protein Is Superior to Peptides
    Authors: Nathali Kaushansky, Nicole Kerlero de Rosbo, Rina Zilkha-Falb, Reut Yosef-Hemo, Lydia Cohen, Avraham Ben-Nun
    PLoS ONE
  40. The non-detergent sulfobetaine-201 acts as a pharmacological chaperone to promote folding and crystallization of the type II TGF-beta receptor extracellular domain
    Authors: Kittikhun Wangkanont, Katrina T. Forest, Laura L. Kiessling
    Protein Expression and Purification
  41. Depletion of TGF-beta from fetal bovine serum
    Authors: Takatoku Oida, Howard L. Weiner
    Journal of Immunological Methods

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Reviews for TGF-beta 1 Biotinylated Antibody

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TGF-beta 1 Biotinylated Antibody
By Anonymous on 03/15/2021
Application: ELISA Sample Tested: Purified protein Species: Human
ELISA TGF-beta 1 Biotinylated Antibody BAF240
TGF-beta 1 Biotinylated Antibody
By Anonymous on 08/29/2019
Application: ELISA Sample Tested: Serum Species: Human
ELISA TGF-beta 1 Biotinylated Antibody BAF240
TGF-beta 1 Biotinylated Antibody
By Anonymous on 03/28/2019
Application: competition ELISA Sample Tested: Recombinant protein Species: Mouse
Other TGF-beta 1 Biotinylated Antibody BAF240
TGF-beta 1 Biotinylated Antibody
By Anonymous on 01/05/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA TGF-beta 1 Biotinylated Antibody BAF240