TGF-beta 1 Antibody

Catalog #: MAB2401
11 Citations Datasheet

Catalog #	Availability	Size / Price	Qty
MAB2401-SP
MAB2401-500
MAB2401-100

All TGF-beta 1 Antibodies

Product Details

Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 1 Antibody Summary

Specificity

Detects multiple species of TGF-beta 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ alpha, rhTGF‑ beta 2, rhTGF‑ beta 3, rhTGF‑ beta 5, rhLAP (TGF‑ beta 1), rhART, rhNeurturin, or recombinant mouse TRANCE.

Source

Monoclonal Mouse IgG_2B Clone # 141322

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TGF-beta 1 (Catalog # 240-B)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Alternate Names

CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for TGF-beta 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells.
Authors: Cai P, Kovacs L, Dong S et al.
Am. J. Physiol. Lung Cell Mol. Physiol.
Suppression of AKT Phosphorylation Restores Rapamycin-Based Synthetic Lethality in SMAD4-Defective Pancreatic Cancer Cells
Authors: Onica Le Le Gendre, Ayisha Sookdeo, Stephie-Anne Duliepre, Matthew Utter, Maria Frias, David A. Foster
Molecular Cancer Research
DCN released from ferroptotic cells ignites AGER-dependent immune responses
Authors: J Liu, S Zhu, L Zeng, J Li, DJ Klionsky, G Kroemer, J Jiang, D Tang, R Kang
Autophagy, 2021-12-29;0(0):1-14.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis.
Authors: Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, Zou B, Xie X, Pasricha P
Mol Pain, 2012-09-11;8(0):65.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization Control
Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB.
Authors: Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J
Arthritis Rheum., 2007-11-01;56(11):3715-25.
Species: Human, Porcine
Sample Types: Whole Cells
Applications: Neutralization
Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
J. Immunol., 2007-04-01;178(7):4184-93.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Authors: Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B
Blood, 2006-02-14;107(11):4424-32.
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Types: Whole Cells
Applications: Neutralization
Nonlymphoid peritoneal cells suppress the T cell response to Mls.
Authors: Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE
Immunobiology, 2004-01-01;209(8):575-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
New methodologies to accurately assess circulating active transforming growth factor-beta 1 levels: implications for evaluating heart failure and the impact of left ventricular assist devices
Authors: Donna Mancini, Juan Monteagudo, Mayte Suárez-Fariñas, Jeffrey Bander, Rohan Varshney, Juana Gonzalez et al.
Translational Research
HIV protease inhibitor-induced cardiac dysfunction and fibrosis is mediated by platelet-derived TGF-?1 and can be suppressed by exogenous carbon monoxide
Authors: J Laurence, S Elhadad, T Robison, H Terry, R Varshney, S Woolington, S Ghafoory, ME Choi, J Ahamed
PLoS ONE, 2017-10-31;12(10):e0187185.

Praliciguat inhibits progression of diabetic nephropathy in ZSF1 rats and suppresses inflammation and apoptosis in human renal proximal tubular cells
Authors: Guang Liu, Courtney M. Shea, Juli E. Jones, Gavrielle M. Price, William Warren, Elisabeth Lonie et al.
American Journal of Physiology-Renal Physiology