TGF-beta 1, 2, 3 Antibody

Catalog #: MAB1835R
5 Citations Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1835R-100
MAB1835R-SP
MAB1835R-500
MAB1835R-01M

TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.

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All TGF-beta 1, 2, 3 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 1, 2, 3 Antibody Summary

Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 1D11R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND₅₀) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody. Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835R). The ND₅₀ is typically0.25-1.25 µg/mL.Catalog # 240-B

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References

Ayala A. et al. (1992) FASEB J. 6:A1604.
Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Long Name

Transforming Growth Factor beta 1, 2, and 3

Alternate Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for TGF-beta 1, 2, 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury
Authors: Tian Zhou, Yiming Zheng, Li Sun, Smaranda Ruxandra Badea, Yuanhu Jin, Yang Liu et al.
Nature Neuroscience
GARP Regulates the Immune Capacity of a Human Autologous Platelet Concentrate
Authors: ER Trzeciak, N Zimmer, PW Kämmerer, D Thiem, B Al-Nawas, A Tuettenber, S Blatt
Biomedicines, 2022-12-05;10(12):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
CD147 Is Essential for the Development of Psoriasis via the Induction of Th17 Cell Differentiation
Authors: A Okubo, Y Uchida, Y Higashi, T Sato, Y Ogawa, A Ryuge, K Kadomatsu, T Kanekura
International Journal of Molecular Sciences, 2021-12-24;23(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis
Authors: N Zimmer, FK Krebs, S Zimmer, H Mitzel-Rin, EJ Kumm, K Jurk, S Grabbe, C Loquai, A Tuettenber
Cancers, 2020-12-05;12(12):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis
Authors: Y Zhao, J Wang, Z Min, L Peng, S Qi, W Lin
Mediators Inflamm., 2020-03-10;2020(0):6947482.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

FAQs

Can the TGF-beta 1, 2, 3 Antibody, Cat# MAB1835, neutralize the activity of all three TGF-beta proteins?
- Yes, this antibody can neutralize the activity of TGF-beta 1, 2, and 3 proteins.

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