TGF-beta Pan Specific Antibody

Catalog # Availability Size / Price Qty
AB-100-NA
TGF-beta Pan Specific in Human Prostate Tissue.
4 Images
Product Details
Citations (91)
FAQs
Supplemental Products
Reviews (3)

TGF-beta Pan Specific Antibody Summary

Specificity
Detects TGF-beta 1, TGF-beta 1.2, TGF-beta 2, TGF-beta 3, and TGF-beta 5 in direct ELISAs and Western blots.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Recombinant human TGF‑ beta 1, porcine platelet-derived TGF‑ beta 1.2, porcine platelet-derived TGF‑ beta 2, and recombinant amphibian TGF‑ beta 5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 2 (Catalog # 302-B2)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
Recombinant Human TGF-beta 3 (Catalog # 243-B3)
Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
under non-reducing conditions only
Immunohistochemistry
3-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is approximately  1 µg/mL, 15 µg/mL, 4 µg/mL, and 1 µg/mL for Porcine TGF-beta 1.2, Porcine TGF-beta 2, Recombinant Chicken TGF-beta 3, and Recombinant Amphibian TGF-beta 5, respectively. The ND50 range for Porcine TGF-beta 1 is 5-30 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TGF-beta Pan Specific antibody in Human Prostate Tissue by Immunohistochemistry (IHC-P). View Larger

TGF-beta Pan Specific in Human Prostate Tissue. TGF-beta was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Rabbit Anti-TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitjhelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta  Antibody. View Larger

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta Antibody. Porcine TGF-beta 1 (Catalog # 101-B1) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND50range is 5-30 µg/mL.

Flow Cytometry Detection of Human TGF-beta by Flow Cytometry View Larger

Detection of Human TGF-beta by Flow Cytometry TGF-beta induces IL-9 mRNA expression in Th1 and Th2 cells.Naïve CD4+ T cells were activated with fibroblast-bound anti-CD3/CD28 under classical Th1 and Th2 conditions, with IL-12 and anti-IL-4 or IL-4, anti-IFN-gamma, and anti-IL-12 respectively for 5 days, restimulated at day 5 and split in cultures with and without TGF-beta or anti-TGF-beta for 5 more days of stimulation. (A, C and D) qRT-PCR gene expression analysis of the relative gene expression of IL-9, GATA3, and Tbet at day 10 relative to the Th0 culture. (B) CT value from qRT-PCR gene expression analysis of the gene PU.1. Each donor is represented by a specific symbol and connected with a line. Data are from four independent experiments, each with two donors. *p<0.05, **p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0021695), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human TGF-beta by Flow Cytometry View Larger

Detection of Human TGF-beta by Flow Cytometry TGF-beta induces IL-9 mRNA expression in Th1 and Th2 cells.Naïve CD4+ T cells were activated with fibroblast-bound anti-CD3/CD28 under classical Th1 and Th2 conditions, with IL-12 and anti-IL-4 or IL-4, anti-IFN-gamma, and anti-IL-12 respectively for 5 days, restimulated at day 5 and split in cultures with and without TGF-beta or anti-TGF-beta for 5 more days of stimulation. (A, C and D) qRT-PCR gene expression analysis of the relative gene expression of IL-9, GATA3, and Tbet at day 10 relative to the Th0 culture. (B) CT value from qRT-PCR gene expression analysis of the gene PU.1. Each donor is represented by a specific symbol and connected with a line. Data are from four independent experiments, each with two donors. *p<0.05, **p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0021695), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Long Name
Transforming Growth Factor beta
Alternate Names
CED; DPD1; LAP; TGFB; TGFB1; TGFbeta; TGF-beta; transforming growth factor beta 1

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Citations for TGF-beta Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

91 Citations: Showing 1 - 10
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  1. The c‑Jun signaling pathway has a protective effect on nucleus pulposus cells in patients with intervertebral disc degeneration
    Authors: Ming Lei, Kun Wang, Shuai Li, Kangcheng Zhao, Wenbin Hua, Xinghuo Wu et al.
    Experimental and Therapeutic Medicine
  2. Sestrin-2, a repressor of PDGFR beta signalling, promotes cigarette-smoke-induced pulmonary emphysema in mice and is upregulated in individuals with COPD
    Authors: Juliana Heidler, Athanasios Fysikopoulos, Frank Wempe, Michael Seimetz, Thorsten Bangsow, Ana Tomasovic et al.
    Disease Models & Mechanisms
  3. TGF-beta Activity Related to the use of Collagen Membranes: In Vitro Bioassays
    Authors: Layla Panahipour, Zahra Kargarpour, Bernadette Luza, Jung-Seok Lee, Reinhard Gruber
    International Journal of Molecular Sciences
  4. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination
    Authors: Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al.
    Nature Neuroscience
  5. The Chronic Stages of Bovine Fasciola hepatica Are Dominated by CD4 T-Cell Exhaustion
    Authors: Divya Sachdev, Kevin C. Gough, Robin J. Flynn
    Frontiers in Immunology
  6. Recombinant Thrombomodulin (Solulin) Ameliorates Early Intestinal Radiation Toxicity in a Preclinical Rat Model
    Authors: Rupak Pathak, Junru Wang, Sarita Garg, Nukhet Aykin-Burns, Karl-Uwe Petersen, Martin Hauer-Jensen
    Radiation Research
  7. Inhibition of TGF-beta 1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-beta 1-Induced EMT in Renal Fibrosis
    Authors: Aurore Devocelle, Lola Lecru, Hélène François, Christophe Desterke, Cindy Gallerne, Pierre Eid et al.
    International Journal of Cell Biology
  8. TGF-beta RI kinase activity mediates Emdogain-stimulated in vitro osteoclastogenesis
    Authors: Reinhard Gruber, Gilles Roos, Jordi Caballé-Serrano, Rick Miron, Dieter D. Bosshardt, Anton Sculean
    Clinical Oral Investigations
  9. Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report
    Authors: Min Jia, Serhiy Souchelnytskyi
    Cell Stress and Chaperones
  10. Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGF beta expression
    Authors: Mahmod Panahi, Naeimeh Yousefi Mesri, Eva‐Britt Samuelsson, Kirsten G. Coupland, Charlotte Forsell, Caroline Graff et al.
    Journal of Cellular and Molecular Medicine
  11. TGF-beta -induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice
    Authors: Parker S. Woods, Mia F. Tazi, Nicholas M. Chesarino, Amal O. Amer, Ian C. Davis
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  12. CD4+CD25+Foxp3+ regulatory T cells suppress NKG2D-mediated NK cell cytotoxicity in peripheral blood
    Authors: Xu Geng, Ming Li, Bin Cui, Chao Lu, Xiaowen Liu, Peng Zhang et al.
    Medicine (Baltimore)
  13. Operational immune tolerance towards transplanted allogeneic pancreatic islets in mice and a non-human primate
    Authors: Midhat H. Abdulreda, Dora M. Berman, Alexander Shishido, Christopher Martin, Maged Hossameldin, Ashley Tschiggfrie et al.
    Diabetologia
  14. UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition
    Authors: Javier Martinez-Useros, Nuria Garcia-Carbonero, Weiyao Li, Maria J. Fernandez-Aceñero, Ion Cristobal, Raul Rincon et al.
    Journal of Clinical Medicine
  15. Extracellular matrix-induced transforming growth factor-beta receptor signaling dynamics
    Authors: N Garamszegi, S P Garamszegi, P Samavarchi-Tehrani, E Walford, M M Schneiderbauer, J L Wrana et al.
    Oncogene
  16. Interleukin-10 deficiency impairs regulatory T cell-derived neuropilin-1 functions and promotes Th1 and Th17 immunity
    Authors: Shimin Wang, Xiang Gao, Guobo Shen, Wei Wang, Jingyu Li, Jingyi Zhao et al.
    Scientific Reports
  17. Th17 Responses to Collagen Type V, k alpha 1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life
    Authors: Jeremy A Sullivan, Ewa Jankowska-Gan, Subramanya Hegde, Matthew A Pestrak, Vrushali V Agashe, Arick C Park et al.
    American Journal of Transplantation
  18. Pentraxin-3 regulates the inflammatory activity of macrophages
    Authors: Aya Shiraki, Norihiko Kotooka, Hiroshi Komoda, Tetsuaki Hirase, Jun-ichi Oyama, Koichi Node
    Biochemistry and Biophysics Reports
  19. Ligand-independent transforming growth factor-beta type I receptor signalling mediates type I collagen-induced epithelial-mesenchymal transition
    Authors: Lucas DeMaio, Stephen T. Buckley, Manda S. Krishnaveni, Per Flodby, Mickael Dubourd, Agnes Banfalvi et al.
    The Journal of Pathology
  20. Potential contribution of immature myeloid CD11c+dendritic cells-derived osteoclast precursor to inflammation-induced bone loss in the TRAF6-null chimeras in-vivo
    Authors: Liu, YCG;Teng, AY;
    Journal of dental sciences
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. Expression of TGF-beta and MMP-2 in hereditary gingival fibromatosis epithelial cells. A possible contribution of the epithelium to its pathogenesis
    Authors: NM Kamal, MA Hamouda, N Abdelgawad
    Journal of oral biology and craniofacial research, 2022-08-14;12(5):617-622.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Inhibition of miR-155 Promotes TGF-beta Mediated Suppression of HIV Release in the Cervical Epithelial Cells
    Authors: J Gokavi, S Sadawarte, A Shelke, U Kulkarni-K, M Thakar, V Saxena
    Viruses, 2021-11-12;13(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. TGF-&beta loaded exosome enhances ischemic wound healing in vitro and in vivo
    Authors: A Shi, J Li, X Qiu, M Sabbah, S Boroumand, TC Huang, C Zhao, A Terzic, A Behfar, SL Moran
    Theranostics, 2021-04-30;11(13):6616-6631.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Effects of Tenascin C on the Integrity of Extracellular Matrix and Skin Aging
    Authors: YE Choi, MJ Song, M Hara, K Imanaka-Yo, DH Lee, JH Chung, ST Lee
    Int J Mol Sci, 2020-11-18;21(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. TGF-&beta in the Secretome of Irradiated Peripheral Blood Mononuclear Cells Supports In Vitro Osteoclastogenesis
    Authors: L Panahipour, Z Kargarpour, M Laggner, M Mildner, HJ Ankersmit, R Gruber
    Int J Mol Sci, 2020-11-13;21(22):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Roxatidine inhibits fibrosis by inhibiting NF?kappaB and MAPK signaling in macrophages sensing breast implant surface materials
    Authors: L Ji, T Wang, L Tian, H Song, M Gao
    Mol Med Rep, 2019-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Cervical cancer cells produce TGF-?1 through the CD73-adenosine pathway and maintain CD73 expression through the autocrine activity of TGF-?1
    Authors: R García-Roc, A Monroy-Gar, J Hernández-, B Weiss-Stei, V Gutiérrez-, M Del Carmen, LR Ávila-Ibar, CA Don-López, DB Torres-Pin, M de Lourdes
    Cytokine, 2018-10-06;118(0):71-79.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  28. DNA methylation affects metastasis of renal cancer and is associated with TGF-?/RUNX3 inhibition
    Authors: J Zheng, Y Mei, P Xiang, G Zhai, N Zhao, C Xu, M Liu, Z Pan, K Tang, D Jia
    Cancer Cell Int., 2018-04-10;18(0):56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Cardiac macrophages promote diastolic dysfunction
    Authors: M Hulsmans, HB Sager, JD Roh, M Valero-Muñ, NE Houstis, Y Iwamoto, Y Sun, RM Wilson, G Wojtkiewic, B Tricot, MT Osborne, J Hung, C Vinegoni, K Naxerova, DE Sosnovik, MR Zile, AD Bradshaw, R Liao, A Tawakol, R Weissleder, A Rosenzweig, FK Swirski, F Sam, M Nahrendorf
    J. Exp. Med., 2018-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  30. The IL-2/Anti-IL-2 Complex Attenuates Cardiac Ischaemia-Reperfusion Injury Through Expansion of Regulatory T Cells
    Authors: J Xiao, K Yu, M Li, C Xiong, Y Wei, Q Zeng
    Cell. Physiol. Biochem., 2017-12-07;44(5):1810-1827.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  31. Naive CD4(+) T Cells Carrying a TLR2 Agonist Overcome TGF-beta-Mediated Tumor Immune Evasion.
    Authors: Ibrahim M, Scozzi D, Toth K, Ponti D, Kreisel D, Menna C, De Falco E, D'Andrilli A, Rendina E, Calogero A, Krupnick A, Gelman A
    J Immunol, 2017-12-06;200(2):847-856.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  32. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017-11-21;12(11):e0187349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Nonmyocyte ERK1/2 signaling contributes to load-induced cardiomyopathy in Marfan mice
    Authors: R Rouf, EG MacFarlane, E Takimoto, R Chaudhary, V Nagpal, PP Rainer, JG Bindman, EE Gerber, D Bedja, C Schiefer, KL Miller, G Zhu, L Myers, N Amat-Alarc, DI Lee, N Koitabashi, DP Judge, DA Kass, HC Dietz
    JCI Insight, 2017-08-03;2(15):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  34. Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-? Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation
    Authors: N Selvamurug, Z He, D Rifkin, B Dabovic, NC Partridge
    Stem Cells Int, 2017-04-23;2017(0):2450327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
    Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
    J. Neurosci., 2017-04-07;0(0):.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Endoplasmic Reticulum Oxidative Stress Triggers Tgf-Beta-Dependent Muscle Dysfunction by Accelerating Ascorbic Acid Turnover
    Authors: D Pozzer, M Favellato, M Bolis, RW Invernizzi, F Solagna, B Blaauw, E Zito
    Sci Rep, 2017-01-20;7(0):40993.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  37. MXRA5 is a TGF-?1-regulated human protein with anti-inflammatory and anti-fibrotic properties
    J Cell Mol Med, 2016-09-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016-08-15;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  39. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions
    Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty
    PLoS ONE, 2016-04-22;11(4):e0153811.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  40. Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells.
    Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A
    PLoS ONE, 2016-01-22;11(1):e0147312.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  41. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015-04-16;112(10):1675-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  42. Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence.
    Authors: Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S
    Nat Commun, 2015-03-09;6(0):6473.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  43. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
    Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J
    Sci Rep, 2015-03-09;5(0):8919.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Blocking
  44. CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice.
    Authors: Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J
    Mol Immunol, 2014-10-12;63(2):420-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  45. Emdogain-regulated gene expression in palatal fibroblasts requires TGF-betaRI kinase signaling.
    Authors: Stahli A, Bosshardt D, Sculean A, Gruber R
    PLoS ONE, 2014-09-08;9(9):e105672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  46. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
    Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
    J Thromb Haemost, 2014-06-27;12(7):1156-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
    Authors: Kang I, Yoon D, Braun K, Wight T
    J Biol Chem, 2014-04-09;289(22):15393-404.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Loss of Dab2 expression in breast cancer cells impairs their ability to deplete TGF-beta and induce Tregs development via TGF-beta.
    Authors: Xu S, Zhu J, Wu Z
    PLoS ONE, 2014-03-17;9(3):e91709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  49. Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
    Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
    Autophagy, 2014-01-01;10(12):2193-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  50. IL-2/anti-IL-2 complex attenuates renal ischemia-reperfusion injury through expansion of regulatory T cells.
    Authors: Kim M, Koo T, Yan J, Lee E, Han K, Jeong J, Ro H, Kim B, Jo S, Oh K, Surh C, Ahn C, Yang J
    J Am Soc Nephrol, 2013-07-05;24(10):1529-36.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  51. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012-11-14;189(12):5590-601.
    Species: Human
    Sample Types: In Vivo
    Applications: Bioassay
  52. Toll-like Receptor 2 (TLR2), Transforming Growth Factor-beta, Hyaluronan (HA), and Receptor for HA-mediated Motility (RHAMM) Are Required for Surfactant Protein A-stimulated Macrophage Chemotaxis.
    Authors: Foley J, Lam D, Jiang H, Liao J, Cheong N, McDevitt T, Zaman A, Wright J, Savani R
    J Biol Chem, 2012-09-04;287(44):37406-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  53. Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis.
    Authors: Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois CM
    Arthritis Res. Ther., 2012-07-04;14(4):R159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  54. Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche.
    Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H
    Cell, 2011-11-23;147(5):1146-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  55. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011-10-17;121(11):4548-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  56. IL-33 induces IL-9 production in human CD4+ T cells and basophils.
    Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
    PLoS ONE, 2011-07-06;6(7):e21695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  57. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010-07-23;33(1):71-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  58. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
    Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
    Circulation, 2009-01-12;119(3):426-35.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  59. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
    Authors: Chase JC, Celli J, Bosio CM
    Infect. Immun., 2008-11-03;77(1):180-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  60. Molecular mechanisms of TGFbeta receptor-triggered signaling cascades rapidly induced by the calcineurin inhibitors cyclosporin A and FK506.
    Authors: Akool el-S, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W
    J. Immunol., 2008-08-15;181(4):2831-45.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  61. Local administration of interleukin-11 ameliorates intestinal radiation injury in rats.
    Authors: Boerma M, Wang J, Burnett AF, Santin AD, Roman JJ, Hauer-Jensen M
    Cancer Res., 2007-10-01;67(19):9501-6.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  62. Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease.
    Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG
    Am. J. Respir. Crit. Care Med., 2007-08-29;176(10):974-82.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  63. Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
    Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
    Am. J. Respir. Cell Mol. Biol., 2007-08-02;38(1):95-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  64. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007-06-06;72(4):481-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  65. Angiotensin II type 1 receptor blockade attenuates TGF-beta-induced failure of muscle regeneration in multiple myopathic states.
    Authors: Cohn RD, van Erp C, Habashi JP, Soleimani AA, Klein EC, Lisi MT, Gamradt M, ap Rhys CM, Holm TM, Loeys BL, Ramirez F, Judge DP, Ward CW, Dietz HC
    Nat. Med., 2007-01-21;13(2):204-10.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  66. Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity.
    Authors: Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T
    Neuroscience, 2006-12-06;144(3):1133-40.
    Species: Rat
    Sample Types: CSF
    Applications: Neutralization
  67. Uptake of host cell transforming growth factor-beta by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion.
    Authors: Waghabi MC, Keramidas M, Bailly S, Degrave W, Mendonca-Lima L, Soeiro Mde N, Meirelles Mde N, Paciornik S, Araujo-Jorge TC, Feige JJ
    Am. J. Pathol., 2005-10-01;167(4):993-1003.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Electron Microscopy, ICC, IHC-P, Neutralization
  68. Nitric oxide induces TIMP-1 expression by activating the transforming growth factor beta-Smad signaling pathway.
    Authors: Akool el-S, Doller A, Muller R, Gutwein P, Xin C, Huwiler A, Pfeilschifter J, Eberhardt W
    J. Biol. Chem., 2005-09-23;280(47):39403-16.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  69. Thrombospondin plays a vital role in the immune privilege of the eye.
    Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW
    Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):908-19.
    Species: Mink, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  70. Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
    Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P
    Neurobiol. Dis., 2005-02-01;18(1):193-205.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  71. Immunoneutralization of growth differentiation factor 9 reveals it partially accounts for mouse oocyte mitogenic activity.
    Authors: Gilchrist RB, Ritter LJ, Cranfield M, Jeffery LA, Amato F, Scott SJ, Myllymaa S, Kaivo-Oja N, Lankinen H, Mottershead DG, Groome NP, Ritvos O
    Biol. Reprod., 2004-05-05;71(3):732-9.
    Species: Bovine, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  72. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004-05-01;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  73. Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
    Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
    J. Immunol., 2004-05-01;172(9):5213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  74. Effect of weightbearing on bone formation during distraction osteogenesis.
    Authors: Leung KS, Cheung WH, Yeung HY, Lee KM, Fung KP
    Clin. Orthop. Relat. Res., 2004-02-01;0(419):251-7.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: IHC-P
  75. Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
    Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J
    Connect. Tissue Res., 2003-01-01;44(1):19-29.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  76. Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.
    Authors: Liu BY, 2019, Wu PW, Bringhurst FR, Wang JT
    141-147, 2002-02-01;143(2):627-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  77. TGF-beta activity protects against inflammatory aortic aneurysm progression and complications in angiotensin II-infused mice
    Authors: Yu Wang, Hafid Ait-Oufella, Olivier Herbin, Philippe Bonnin, Bhama Ramkhelawon, Soraya Taleb et al.
    Journal of Clinical Investigation
  78. Effect of seminal plasma or transforming growth factor on bovine endometrial cells
    Authors: Jason A Rizo, Laila A Ibrahim, Paula C C Molinari, Bo R Harstine, Rachel L Piersanti, John J Bromfield
    Reproduction
  79. Excessive vascular sprouting underlies cerebral hemorrhage in mice lacking alpha V beta 8-TGF beta signaling in the brain
    Authors: Thomas D. Arnold, Colin Niaudet, Mei-Fong Pang, Julie Siegenthaler, Konstantin Gaengel, Bongnam Jung et al.
    Development
  80. Activation of human vascular cells decreases their expression of transforming growth factor-beta
    Authors: Amir H. Lebastchi, Lingfeng Qin, Salman F. Khan, Jing Zhou, Arnar Geirsson, Richard W. Kim et al.
    Atherosclerosis
  81. Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model
    Authors: TR Karhadkar, D Pilling, N Cox, RH Gomer
    Sci Rep, 2017-11-08;7(1):15069.
  82. TGF-beta Plays a Key Role in Morphogenesis of the Pancreatic Islets of Langerhans by Controlling the Activity of the Matrix Metalloproteinase MMP-2
    Authors: Francisco Miralles, Tadej Battelino, Paul Czernichow, Raphael Scharfmann
    J. Cell Biol
  83. Transforming Growth Factor Beta Expression by Human Vascular Cells Inhibits Interferon Gamma Production and Arterial Media Injury by Alloreactive Memory T Cells
    Authors: Amir H. Lebastchi, Salman F. Khan, Lingfeng Qin, Wei Li, Jing Zhou, Narutoshi Hibino et al.
    American Journal of Transplantation
  84. Infusion of Bone Marrow Mononuclear Cells Reduces Lung Fibrosis but Not Inflammation in the Late Stages of Murine Silicosis
    Authors: Miquéias Lopes-Pacheco, Túlio G. Ventura, Helena D'Anunciação de Oliveira, Leonardo C. Monção-Ribeiro, Bianca Gutfilen, Sergio A. L. de Souza et al.
    PLoS ONE
  85. Tumor-supportive and osteoclastogenic changes induced by breast cancer-derived factors are reversed by inhibition of {gamma}-secretase
    Authors: Jenna E. Fong, Damien Le Nihouannen, Svetlana V. Komarova
    Journal of Biological Chemistry
  86. The Controversial Role of TGF-beta in Neovascular Age-Related Macular Degeneration Pathogenesis
    Authors: Gian Marco Tosi, Maurizio Orlandini, Federico Galvagni
    International Journal of Molecular Sciences
  87. Disruption of LTBP-4 function reduces TGF-beta activation and enhances BMP-4 signaling in the lung
    Authors: Katri Koli, Frank Wempe, Anja Sterner-Kock, Anna Kantola, Martina Komor, Wolf-K. Hofmann et al.
    The Journal of Cell Biology
  88. Donor HLA−DR Drives the Development of De Novo Autoimmunity Following Lung and Heart Transplantation
    Authors: Ewa Jankowska−Gan, Vrushali V. Agashe, Diego A. Lema, Ying Zhou, Laura Gonzalez Bosc, Jeremy A. Sullivan et al.
    Transplantation Direct
  89. Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress-mediated cell death pathways
    Authors: TSAI-KUN WU, CHYOU-WEI WEI, YING-RU PAN, SHUR-HUEIH CHERNG, WEI-JUNG CHANG, HSUEH-FANG WANG et al.
    Molecular Medicine Reports
  90. Micellar Casein and Whey Powder Hold a TGF-beta Activity and Regulate ID Genes In Vitro
    Authors: Layla Panahipour, Selma Husejnovic, Jila Nasirzade, Stephan Semelmayer, Reinhard Gruber
    Molecules
  91. A protective role for periostin and TGF ‐ beta in IgE ‐mediated allergy and airway hyperresponsiveness
    Authors: E. D. Gordon, S. S. Sidhu, Z.‐E. Wang, P. G. Woodruff, S. Yuan, M. C. Solon et al.
    Clinical & Experimental Allergy

FAQs

  1. Does TGF-beta Pan Specific Antibody, catalog # AB-100-NA, recognize active or latent TGF-beta (LAP)?

    • This antibody recognizes mature (active) TGF-beta. We have not tested for cross-reactivity to LAP (catalog # 246-LP) in our neutralization assay. Some cross-reactivity to latent TGF-beta may also be observed in western blot.

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TGF-beta Pan Specific Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse oral tissue Species: Mouse

TGF-beta Pan Specific Antibody
By Anonymous on 03/23/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

TGF-beta Pan Specific Antibody
By Anonymous on 06/06/2017
Application: B/N Sample Tested: Peritoneal macrophages Species: Mouse