TGF-beta 1 Magnetic Luminex® Performance Assay

Catalog #: LTGM100
8 Citations Datasheet

Build your own Luminex Assay with our Luminex Assay Customization Tool.

U.S. orders usually ship in 2-3 business days

Build Now

Quantist software graph of signal intensity versus concentration for TGF-beta 1 in the Human TGF-beta Luminex Panel

1 Image

All TGF-beta 1 Luminex Assays

Product Details

Citations (8)

FAQs

Supplemental Products

Reviews

Summary Configure Your Assay Product Features Precision & Accuracy Data Examples Product Datasheets Background

TGF-beta 1 Magnetic Luminex® Performance Assay Summary

Panel	Luminex Performance Human TGF-beta Panel
Assay Type	Magnetic bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and Magnetic antibody-coated beads
Species	Human, Mouse, Rat, Porcine
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human TGF-beta 1 with up to analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine^® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Base Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents

User-mix antibody-coated Magnetic beads in individual vials
User-mixed cocktail of biotinylated detection antibody

Precision

	Intra-Assay Precision			Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in ninety-four separate assays to assess precision between assays.
Sample	1	2	3	1	2	3
n	20	20	20	94	94	94
Mean (pg/mL)	166	762	4422	158	837	5122
Standard deviation	11	37	276	30	130	842
CV (%)	6.6	4.9	6.2	19.0	15.5	16.4

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human TGF-B1 were evaluated for recovery.
Cell culture supernate	100	72-119
Serum	97	64-115
EDTA plasma	100	78-126
Heparin plasma	97	77-117
Platelet-poor EDTA plasma	103	86-118
Platelet-poor heparin plasma	104	87-122
Urine	103	89-120

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma	Platelet-poor EDTA plasma	Platelet-poor Heparin plasma	Urine
Samples containing and/or spiked with human TGF-B1 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	101	96	99	98	109	103	102
1:2	Range (%)	75-123	88-109	94-110	92-103	102-113	99-109	92-111
1:4	Average % of Expected	99	92	100	99	103	99	94
1:4	Range (%)	76-120	80-103	90-116	80-119	94-115	92-104	84-104
1:8	Average % of Expected	94	94	96	99	99	95	89
1:8	Range (%)	64-116	84-101	82-112	79-125	81-120	86-107	71-107

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex^® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad^® Bio-Plex^®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

N/A

View Larger

Quantist software graph of signal intensity versus concentration for TGF-beta 1 in the Human TGF-beta Luminex Panel

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Alternate Names

CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Product Datasheets

Product Specific Notices

Luminex® is a registered trademark of Luminex Corporation.

Citations for TGF-beta 1 Magnetic Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

Differences in the Cellular Immune Response during and after Treatment of Sudanese Patients with Post-kala-azar Dermal Leishmaniasis, and Possible Implications for Outcome
Authors: Torres, A;Younis, BM;Alamin, M;Tesema, S;Bernardo, L;Solana, JC;Moreno, J;Mustafa, AA;Alves, F;Musa, AM;Carrillo, E;
Journal of epidemiology and global health
Species: Human
Sample Types: Plasma
Integrin/TGF-beta1 inhibitor GLPG-0187 blocks SARS-CoV-2 Delta and Omicron pseudovirus infection of airway epithelial cells which could attenuate disease severity
Authors: KE Huntington, L Carlsen, EY So, M Piesche, O Liang, WS El-Deiry
Oncogene, 2022-01-03;0(0):.
Species: Human
Sample Types: Plasma
Higher CD19+CD25+ Bregs are independently associated with better graft function in renal transplant recipients
Authors: EH Ibrahim, MG Aly, G Opelz, C Morath, M Zeier, C Süsal, DM Sayed, E Hassan, N Ekpoom, V Daniel
Bmc Nephrology, 2021-05-17;22(1):180.
Species: Human
Sample Types: Plasma
The Exposure to Osteoarthritic Synovial Fluid Enhances the Immunomodulatory Profile of Adipose Mesenchymal Stem Cell Secretome
Authors: A Cifù, R Domenis, M Pozzi-Muce, P Di Benedet, A Causero, M Moretti, M Stevanato, C Pistis, PC Parodi, M Fabris, F Curcio
Stem Cells Int, 2020-07-18;2020(0):4058760.
Species: Human
Sample Types: Cell Culture Supernates
Inhibition of bone morphogenetic protein 6 receptors ameliorates Sjï¿½gren's syndrome in mice
Authors: H Yin, L Kalra, Z Lai, MC Guimaro, L Aber, BM Warner, D Michael, N Zhang, J Cabrera-Pe, A Karim, WD Swaim, S Afione, A Voigt, CQ Nguyen, PB Yu, DB Bloch, JA Chiorini
Sci Rep, 2020-02-19;10(1):2967.
Species: Mouse
Sample Types: Serum
Multiple cytokine analyses of aqueous humor from the patients with retinitis pigmentosa
Authors: B Lu, H Yin, Q Tang, W Wang, C Luo, X Chen, X Zhang, K Lai, J Xu, X Chen, K Yao
Cytokine, 2019-12-03;127(0):154943.
Species: Human
Sample Types: Aqueous Humor
Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes
Authors: R Domenis, A Cifù, D Marinò, M Fabris, KR Niazi, P Soon-Shion, F Curcio
Sci Rep, 2019-06-11;9(1):8457.
Species: Human
Sample Types: Whole Cells
Cytokines in relation to hCG are significantly altered in asymptomatic women with miscarriage - a pilot study
Authors: A Freis, J Schlegel, V Daniel, J Jauckus, T Strowitzki, A Germeyer
Reprod. Biol. Endocrinol., 2018-09-28;16(1):93.
Species: Human
Sample Types: Plasma