TACS XTT Cell Proliferation Assay

Catalog #: 4891-025-K
30 Citations 3 Reviews Datasheet

A sensitive kit for the measurement of cell proliferation using XTT

Catalog #	Availability	Size / Price	Qty
4891-025-K

TACS XTT Cell Proliferation/Viability Assay_4891-025-K

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Citations (30)

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Reviews (3)

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TACS XTT Cell Proliferation Assay Summary

Provides a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide.

Key Benefits

• Allows measurement of cell proliferation, cell viability or cytotoxicity.
• Rapid determination in microplates (no solubilization step as in an MTT assay).
• Reproducible and sensitive results.
• No radioactivity.
• Ideal for high throughput assays (no washing or other steps that can cause cell loss and variability).

Why Use the TACS XTT Cell Proliferation Assay?

The XTT Cell Proliferation Assay (XTT-CPA) is a sensitive kit for the measurement of cell proliferation. Cleavage of the tetrazolium salt to formazan occurs via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells.

Kit Contents

• XTT Reagent
• XTT Activator

Specifications

Shipping Conditions

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Species

N/A

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet

Scientific Data

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Relative Cell Number Measured using the TACS XTT Cell Proliferation/Viability Assay. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densities. Cells were incubated with XTT for 6 hours at 27 °C and absorbance was read using an ELISA plate reader.

Citations for TACS XTT Cell Proliferation Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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Immunomodulatory Role of Rosmarinus officinalis L., Mentha x piperita L., and Lavandula angustifolia L. Essential Oils in Sheep Peripheral Blood Mononuclear Cells
Authors: Ciliberti, MG;Albenzio, M;Sevi, A;Frabboni, L;Marino, R;Caroprese, M;
Veterinary sciences 2024-04-01
Epigenetic histone modification by butyrate downregulates KIT and attenuates mast cell function
Authors: Gudneppanavar, R;Sabu Kattuman, EE;Teegala, LR;Southard, E;Tummala, R;Joe, B;Thodeti, CK;Paruchuri, S;
Journal of cellular and molecular medicine 2023-08-21
Chitosan Grafted with Thermoresponsive Poly(di(ethylene glycol) Methyl Ether Methacrylate) for Cell Culture Applications
Authors: N Dasgupta, D Sun, M Gorbet, M Gauthier
Polymers, 2023-03-18;15(6):. 2023-03-18
Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 Pathway
Authors: P Banerjee, S Kumaravel, S Roy, N Gaddam, J Odeh, KJ Bayless, S Glaser, S Chakrabort
Cells, 2023-02-06;12(4):. 2023-02-06
Evaluation of the antioxidant potency of Greek honey from the Taygetos and Pindos mountains using a combination of cellular and molecular methods
Authors: A Patouna, P Vardakas, Z Skaperda, DA Spandidos, D Kouretas
Molecular Medicine Reports, 2023-01-20;27(2):. 2023-01-20
Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling
Authors: P Melo, G Montalbano, E Boggio, CL Gigliotti, C Dianzani, U Dianzani, C Vitale-Bro, S Fiorilli
Polymers, 2022-09-09;14(18):. 2022-09-09
The long noncoding RNA H19 regulates tumor plasticity in neuroendocrine prostate cancer
Authors: N Singh, VR Ramnarine, JH Song, R Pandey, SKR Padi, M Nouri, V Olive, M Kobelev, K Okumura, D McCarthy, MM Hanna, P Mukherjee, B Sun, BR Lee, JB Parker, D Chakravart, NA Warfel, M Zhou, JJ Bearss, EA Gibb, M Alshalalfa, RJ Karnes, EJ Small, R Aggarwal, F Feng, Y Wang, R Buttyan, A Zoubeidi, M Rubin, M Gleave, FJ Slack, E Davicioni, H Beltran, C Collins, AS Kraft
Nature Communications, 2021-12-21;12(1):7349. 2021-12-21
Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury
Authors: D Medica, R Franzin, A Stasi, G Castellano, M Migliori, V Panichi, F Figliolini, L Gesualdo, G Camussi, V Cantaluppi
Cells, 2021-07-02;10(7):. 2021-07-02
Anti-inflammatory activity of Nectandra angustifolia (Laurel Amarillo) ethanolic extract
Authors: L Ferrini, JP Rodríguez, JP Melana Col, G Olea, GA Ojeda, G Ricciardi, AM Torres, MV Aguirre
Journal of ethnopharmacology, 2021-02-18;272(0):113937. 2021-02-18
Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells
Authors: CH Yeh, M Bellon, F Wang, H Zhang, L Fu, C Nicot
Mol. Cancer, 2020-09-09;19(1):139. 2020-09-09

FAM83G Is a Novel Inducer of Apoptosis
Authors: J Okada, N Sunaga, E Yamada, T Saito, A Ozawa, Y Nakajima, K Okada, JE Pessin, S Okada, M Yamada
Molecules, 2020-06-18;25(12):. 2020-06-18

Transient receptor potential ion channel TRPM2 promotes AML proliferation and survival through modulation of mitochondrial function, ROS, and autophagy
Authors: SJ Chen, L Bao, K Keefer, S Shanmughap, L Chen, J Lee, J Wang, XQ Zhang, I Hirschler-, S Merali, C Merali, Y Imamura, S Dovat, M Madesh, JY Cheung, HG Wang, BA Miller
Cell Death Dis, 2020-04-20;11(4):247. 2020-04-20

The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment
Authors: E Kim, E Ten Hacken, M Sivina, A Clarke, PA Thompson, N Jain, A Ferrajoli, Z Estrov, MJ Keating, WG Wierda, KN Bhalla, JA Burger
Leukemia, 2019-12-20;0(0):. 2019-12-20

The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2
Authors: L Bao, F Festa, CS Freet, JP Lee, IM Hirschler-, SJ Chen, KA Keefer, HG Wang, AD Patterson, JY Cheung, BA Miller
Sci Rep, 2019-10-01;9(1):14132. 2019-10-01

Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells
Authors: HM Tunset, AJ Feuerherm, LM Selvik, B Johansen, SA Moestue
Int J Mol Sci, 2019-09-27;20(19):. 2019-09-27

Hepatoprotective effect of Solanum surattense leaf extract against chemical- induced oxidative and apoptotic injury in rats
Authors: MK Parvez, MS Al-Dosari, AH Arbab, P Alam, MS Alsaid, AA Khan
BMC Complement Altern Med, 2019-07-03;19(1):154. 2019-07-03

Potent and selective EGFR inhibitors based on 5-aryl-7H-pyrrolopyrimidin-4-amines
Authors: AC Reiersølmo, TI Aarhus, S Eckelt, KG Nørsett, E Sundby, BH Hoff
Bioorg. Chem., 2019-04-11;88(0):102918. 2019-04-11

Proanthocyanidins from the stem bark of Rhus tripartita ameliorate methylgloxal-induced endothelial cell apoptosis
Authors: AS Alqahtani, WM Abdel-Mage, AA Shahat, MK Parvez, MS Al-Dosari, A Malik, MS Abdel-Kade, MS Alsaid
J Food Drug Anal, 2019-03-17;27(3):758-765. 2019-03-17

Drug Repositioning Inferred from E2F1-Coregulator Interactions Studies for the Prevention and Treatment of Metastatic Cancers
Authors: D Goody, SK Gupta, D Engelmann, A Spitschak, S Marquardt, S Mikkat, C Meier, C Hauser, JP Gundlach, JH Egberts, H Martin, T Schumacher, A Trauzold, O Wolkenhaue, S Logotheti, BM Pützer
Theranostics, 2019-02-20;9(5):1490-1509. 2019-02-20

Intraocular DHODH-inhibitor PP-001 suppresses relapsing experimental uveitis and cytokine production of human lymphocytes, but not of RPE cells
Authors: M Diedrichs-, S Niesik, CS Priglinger, SR Thurau, F Obermayr, S Sperl, G Wildner
J Neuroinflammation, 2018-02-21;15(1):54. 2018-02-21

Stabilization of telomere G-quadruplexes interferes with human herpesvirus 6A chromosomal integration
Authors: S Gilbert-Gi, A Gravel, S Artusi, SN Richter, N Wallaschek, BB Kaufer, L Flamand
J. Virol., 2017-06-26;0(0):. 2017-06-26

Methotraxate-Loaded Hybrid Nanoconstructs Target Vascular Lesions and Inhibit Atherosclerosis Progression in ApoE<sup>-/-</sup> Mice.
Authors: Stigliano C, Ramirez M, Singh J, Aryal S, Key J, Blanco E, Decuzzi P
Adv Healthc Mater, 2017-04-12;6(13):. 2017-04-12

Anti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3Kdelta/gamma.
Authors: Davis R, Moore E, Clavijo P, Friedman J, Cash H, Chen Z, Silvin C, Van Waes C, Allen C
Cancer Res, 2017-03-31;77(10):2607-2619. 2017-03-31

Blockade of CCR5 receptor prevents M2 microglia phenotype in a microglia-glioma paradigm.
Authors: Laudati E, Curro D, Navarra P, Lisi L
Neurochem Int, 2017-03-06;108(0):100-108. 2017-03-06

Resetting microbiota by Lactobacillus reuteri inhibits T reg deficiency-induced autoimmunity via adenosine A2A receptors.
Authors: He B, Hoang T, Wang T, Ferris M, Taylor C, Tian X, Luo M, Tran D, Zhou J, Tatevian N, Luo F, Molina J, Blackburn M, Gomez T, Roos S, Rhoads J, Liu Y
J Exp Med, 2016-12-19;214(1):107-123. 2016-12-19

Depletion of the Human Ion Channel TRPM2 in Neuroblastoma Demonstrates Its Key Role in Cell Survival through Modulation of Mitochondrial Reactive Oxygen Species and Bioenergetics.
Authors: Bao L, Chen S, Conrad K, Keefer K, Abraham T, Lee J, Wang J, Zhang X, Hirschler-Laszkiewicz I, Wang H, Dovat S, Gans B, Madesh M, Cheung J, Miller B
J Biol Chem, 2016-09-30;291(47):24449-24464. 2016-09-30

A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2alpha.
Authors: Chen S, Hoffman N, Shanmughapriya S, Bao L, Keefer K, Conrad K, Merali S, Takahashi Y, Abraham T, Hirschler-Laszkiewicz I, Wang J, Zhang X, Song J, Barrero C, Shi Y, Kawasawa Y, Bayerl M, Sun T, Barbour M, Wang H, Madesh M, Cheung J, Miller B
J Biol Chem, 2014-11-12;289(52):36284-302. 2014-11-12

Akt-activated endothelium constitutes the niche for residual disease and resistance to bevacizumab in ovarian cancer.
Authors: Guerrouahen B, Pasquier J, Kaoud N, Maleki M, Beauchamp M, Yasmeen A, Ghiabi P, Lis R, Vidal F, Saleh A, Gotlieb W, Rafii S, Rafii A
Mol Cancer Ther, 2014-10-15;13(12):3123-36. 2014-10-15

Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells.
Authors: Hagelgans A, Nacke B, Zamaraeva M, Siegert G, Menschikowski M
Anticancer Res, 2014-04-01;34(4):1723-9. 2014-04-01

Nucleobindin-2 is a positive modulator of EGF-dependent signals leading to enhancement of cell growth and suppression of adipocyte differentiation.
Authors: Tagaya Y, Miura A, Okada S, Ohshima K, Mori M
Endocrinology, 2012-04-18;153(7):3308-19. 2012-04-18