Streptavidin Antibody

Catalog #: MAB9020 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9020
MAB9020-SP

Detection of Streptavidin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Streptavidin Antibody Summary

Specificity

Detects Streptavidin in Western blot.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1220C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Purified streptavidin from Streptomyces avidinii

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Streptavidin by Western Blot. Western blot shows recombinant Streptavidin. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Streptavidin Monoclonal Antibody (Catalog # MAB9020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Streptavidin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Streptavidin

Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (K_d) on the order of ≈10⁻¹⁴ mol/L. The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2; Streptavidin

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