Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge

Catalog #: SPCKB-MP-008305 Datasheet
Simple Plex Mouse/Rat VCAM-1/CD106 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-MP-008305
SPCKA-MP-008305
SPCKC-MP-008305
Control Products Available
Mouse/Rat VCAM-1/CD106 Ella Assay
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Simple Plex Mouse/Rat VCAM-1/CD106 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
1.59 pg/mL
Assay Range
8.0 - 30,372 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
natural mouse and rat and recombinant mouse VCAM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat VCAM-1 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 44 43
Mean (pg/mL) 197 9548 198 10414
Standard Deviation 8.38 546 21.2 1216
CV% 4.2 5.7 10.7 11.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 105 103-107

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat VCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Mouse VCAM-1/CD106 Ella Assay Linearity
Rat VCAM-1/CD106 Ella Assay Linearity

Scientific Data

Mouse/Rat VCAM-1/CD106 Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VCAM-1/CD106

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.

Long Name:
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs:
7412 (Human); 22329 (Mouse)
Alternate Names:
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

FAQs

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