Simple Plex Mouse/Rat Angiopoietin-2 Cartridge

Catalog #: SPCKB-MP-003636 Datasheet

Simple Plex Mouse/Rat Angiopoietin-2/ANG-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-003636
SPCKA-MP-003636
SPCKC-MP-003636

Control Products Available

896173: Simple Plex Control for Mouse/Rat Angiopoietin-2

Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma/Serum.

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All Angiopoietin-2 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat Angiopoietin-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 pg/mL), Serum (5 pg/mL), EDTA Plasma (5 pg/mL), Heparin Plasma (5 pg/mL)

Sensitivity

1.1 pg/mL

Assay Range

10.5 - 40,130 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse and rat Angiopoietin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse/rat Angiopoietin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	93	89
Mean (pg/mL)	198	10863	240	11457
Standard Deviation	18.6	452	27.5	1311
CV%	9.4	4.2	11.5	11.4

Recovery

Recovery was not necessary as Angiopoietin-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Angiopoietin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Rat Angiopoietin-2 Simple Plex Assay Linearity

Scientific Data

N/A

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Mouse/Rat Angiopoietin-2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:

285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)

Alternate Names:

AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand