Simple Plex Mouse IL-5 Cartridge

Catalog #: SPCKB-MP-002846 Datasheet

Simple Plex Mouse IL-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-002846
SPCKA-MP-002846
SPCKC-MP-002846

Control Products Available

898642: Simple Plex Control for Mouse IL-5

Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma/Serum.

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse IL-5 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (10 uL), Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)

Sensitivity

0.17 pg/mL

Assay Range

0.5 - 4,290 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Specificity

Natural and recombinant mouse IL-5.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse IL-5 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	46
Mean (pg/mL)	28.3	1593	24.8	1537
Standard Deviation	1.65	107	2.23	127
CV%	5.8	6.7	9	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=5)	109	98-120
Mouse Heparin Plasma (n=5)	107	98-111
Mouse Serum (n=3)	106	101-115

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Mouse IL-5 Simple Plex Assay Standard Curve

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Mouse IL-5 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Long Name:

Interleukin 5

Entrez Gene IDs:

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names:

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I