Simple Plex Mouse Granzyme B Cartridge

Catalog #: SPCKB-MP-007963 Datasheet

Simple Plex Mouse Granzyme B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-007963
SPCKA-MP-007963
SPCKC-MP-007963

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All Granzyme B Simple Plex Assays

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse Granzyme B Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Specificity

Natural and recombinant mouse Granzyme B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse Granzyme B in cell culture supernatant (CCS), serum, and EDTA plasma.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Linearity

Samples containing and/or spiked with high concentrations of mouse Granzyme B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Mouse Granzyme B Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Entrez Gene IDs:

3002 (Human); 14939 (Mouse)

Alternate Names:

C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT