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Simple Plex Human VEGFR1/Flt-1 Cartridge

Catalog #: SPCKB-PS-000540 Datasheet
Simple Plex Human VEGFR1/Flt-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000540
SPCKA-PS-000540
SPCKC-PS-000540
Human VEGFR1/Flt-1 Ella Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human VEGFR1/Flt-1 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.6 pg/mL
Assay Range
3.1 - 4,650 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human VEGF R1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Vascular Endothelial Growth Factor Receptor 1 (VEGF R1/Flt1) in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 19
Mean (pg/mL) 27.1 1312 28.3 1336
Standard Deviation 1.99 123 1.99 119
CV% 7.3 9.4 7 8.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=3) 104 99-111
Heparin Plasma (n=3) 81 73-88
Serum (n=3) 97 79-111

Linearity

Samples containing and/or spiked with high concentra-tions of human VEGF R1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human VEGFR1/Flt-1 Ella Assay Linearity

Scientific Data

N/A View Larger

Human VEGFR1/Flt-1 Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long Name:
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs:
2321 (Human); 14254 (Mouse)
Alternate Names:
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

FAQs

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