Simple Plex Human u-Plasminogen Activator Cartridge

Catalog #: SPCKB-PS-000538 Datasheet
Simple Plex Human u-Plasminogen Activator/uPA/Urokinase assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000538
SPCKA-PS-000538
SPCKC-PS-000538
Control Products Available
Human uPA Simple Plex Assay
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Simple Plex Human u-Plasminogen Activator Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)
Sensitivity
1.33 pg/mL
Assay Range
3.9 - 15,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human uPA.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Neurogranin in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and urine. 

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 21
Mean (pg/mL) 70.7 3769 72.3 3684
Standard Deviation 3.4 257 10.2 413
CV% 4.8 6.8 14.1 11.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 99 97-100

Linearity

Samples containing and/or spiked with high concentrations of human uPA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human uPA Ella Assay Linearity

Scientific Data

Human uPA Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: u-Plasminogen Activator (uPA)/Urokinase

u-Plasminogen Activator (uPA) is a serine protease that converts plasminogen to plasmin, with roles in a variety of normal and pathological processes that include cell migration and tissue destruction. uPA is a potent marker of invasion and metastasis in a variety of human cancers including breast, stomach, colon, bladder, ovarian, brain, and endometrium.

Long Name:
Urokinase-type Plasminogen Activator
Entrez Gene IDs:
5328 (Human); 18792 (Mouse); 102135886 (Cynomolgus Monkey)
Alternate Names:
ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator

FAQs

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