Simple Plex Human Pentraxin 3/TSG-14 Cartridge

Catalog #: SPCKB-PS-000537 Datasheet

Simple Plex Human Pentraxin 3/TSG-14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000537
SPCKA-PS-000537
SPCKC-PS-000537

Control Products Available

898213: Simple Plex Control for Human Pentraxin 3/TSG-14

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Pentraxin 3/TSG-14 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)

Sensitivity

2.26 pg/mL

Assay Range

5.2 - 32,530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human Pentraxin 3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Pentraxin 3 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and saliva.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	17
Mean (pg/mL)	206	9568	211	9837
Standard Deviation	10.1	46	11.7	552
CV%	4.9	4.8	5.5	5.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	105	103-110
EDTA Plasma (n=4)	98	92-109
Heparin Plasma (n=4)	102	98-109

Linearity

Samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Pentraxin 3/TSG-14 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:

Tumor Necrosis Factor Stimulated Gene 14

Entrez Gene IDs:

5806 (Human); 19288 (Mouse)

Alternate Names:

alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Related Research Areas

FAQs

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