Simple Plex Human PDGF-BB Cartridge

Catalog #: SPCKB-PS-000283 Datasheet

Simple Plex Human PDGF-BB assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000283
SPCKA-PS-000283
SPCKC-PS-000283

Control Products Available

894973: Simple Plex Control for Human PDGF-BB

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All PDGF-BB Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human PDGF-BB Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)

Sensitivity

0.291 pg/mL

Assay Range

0.8 - 1,200 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant human PDGF-BB.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human PDGF-BB in serum and platelet-poor plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	7	6
Mean (pg/mL)	7.2	294	6.92	297
Standard Deviation	0.39	15.7	0.392	18.8
CV%	5.4	5.3	5.7	6.3

Recovery

Recovery was not necessary as PDGF-BB shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human PDGF-BB were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human PDGF-BB Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:

Platelet-derived Growth Factor BB

Entrez Gene IDs:

5155 (Human); 24628 (Rat)

Alternate Names:

c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB