Simple Plex Human Neprilysin/CD10 Cartridge

Catalog #: SPCKB-PS-001519 Datasheet

Simple Plex Human Neprilysin/CD10 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001519
SPCKA-PS-001519
SPCKC-PS-001519

Control Products Available

898987: Simple Plex Control for Human Neprilysin/CD10

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Neprilysin/CD10 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.82 pg/mL

Assay Range

11.8 - 18,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Neprilysin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Neprilysin in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	56	59
Mean (pg/mL)	114	5616	123	6348
Standard Deviation	6.41	326	11.8	611
CV%	5.6	5.8	9.6	9.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	100	96-103
Heparin Plasma (n=4)	97	91-103
Serum (n=4)	96	93-100

Linearity

Samples containing and/or spiked with high concentrations of human Neprilysin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human Neprilysin Simple Plex Assay Standard Curve

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Human Neprilysin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neprilysin/CD10

The neprilysin (NEP) family of zinc metallopeptidases includes neprilysin, endothelin-converting enzyme-2 (ECE-2), PEX, damage induced neuronal endopeptidase (DINE), Kell and several neprilysin-like proteins. Neprilysin is expressed at the cell surface of a variety of cell types. Enzymatically, neprilysin functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. Neprilysin has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide. Neprilysin has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states. These and other observations have resulted in considerable clinical interest in neprilysin as a potential target for analgesics and antihypertensive drugs. Neprilysin is also a major degrading enzyme of amyloid-beta peptide (A-beta) in the brain, indicating that down-regulation of neprilysin activity, which could be caused by aging, can contribute to the development of Alzheimers disease by promoting A-beta accumulation.

Entrez Gene IDs:

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Alternate Names:

Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase