Simple Plex Human IL-7 Cartridge

Catalog #: SPCKB-PS-000506 Datasheet

Simple Plex Cartridge Kit for quantitation of IL-7. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000506
SPCKA-PS-000506
SPCKC-PS-000506

Control Products Available

894969: Simple Plex Control for Human IL-7

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IL-7 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.08 pg/mL

Assay Range

0.6 - 2,170 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-7.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-7 in cell culture supernatan (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	1
n	16	16	12	12
Mean (pg/mL)	11.9	622	12	628
Standard Deviation	0.454	35.5	0.58	28.7
CV%	3.8	5.7	4.8	4.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	93	91-98
EDTA Plasma (n=4)	95	90-102
Heparin Plasma (n=4)	76	70-84
Serum (n=4)	96	90-101

Linearity

Samples containing and/or spiked with high concentrations of human IL-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human IL-7 Simple Plex Assay Standard Curve

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Human IL-7 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Long Name:

Interleukin 7

Entrez Gene IDs:

3574 (Human); 16196 (Mouse); 25647 (Rat)

Alternate Names:

IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF