Simple Plex Human IgG Pan Specific Cartridge New

Catalog #: SPCKB-PS-007587 Datasheet

Simple Plex Human IgG Pan Specific assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD19, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKC-PS-007587
SPCKA-PS-007587
SPCKB-PS-007587

Control Products Available

897686: Simple Plex Control for Human IgG Pan

Simple Plex Human IgG Pan Specific Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IgG Pan Specific Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

329 pg/mL

Assay Range

371.0 - 90,560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IgG Pan Specific.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human IgG Pan Specific in serum and plasma (EDTA/Heparin) in Sample Diluent SD19.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IgG Pan Specific assay

Inter-Assay Precision (Precision between assays) Replicates of each IgG Pan Specific control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IgG Pan Specific

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	53	54
Mean (pg/mL)	848	40300	662	37163
Standard Deviation	41	846	82	2781
CV%	4.9	2.1	12.3	7.5

Recovery

Recovery was not necessary as human IgG Pan Specific shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of IgG Pan Specific were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IgG Pan Specific Assay. Assay range is 371-90,560 pg/mL.

Product Datasheets

Product Datasheet

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name:

Immunoglobulin G

Alternate Names:

IgG; Immunoglobulin G; ImmunoglobulinG

FAQs

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