Simple Plex Human CXCL12/SDF-1 alpha Cartridge

Catalog #: SPCKB-PS-000299 Datasheet

Simple Plex Human CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000299
SPCKA-PS-000299
SPCKC-PS-000299

Control Products Available

898082: Simple Plex Control for Human CXCL12/SDF-1 alpha

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All CXCL12/SDF-1 alpha Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Platelet-poor EDTA Plasma (5 uL), Platelet-poor Heparin Plasma (5 uL)

Sensitivity

6.57 pg/mL

Assay Range

6.0 - 1,476 pg/mL (Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant human SDF-1 alpha.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human SDF-1 alpha in platelet-poor plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	41
Mean (pg/mL)	27.1	1221	22.7	1049
Standard Deviation	1.56	66.5	3.05	77.4
CV%	5.8	5.4	13.4	7.4

Recovery

Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human SDF-1 alpha Simple Plex Assay Standard Curve

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Human SDF-1 alpha Ella Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Entrez Gene IDs:

6387 (Human); 20315 (Mouse); 493806 (Feline)

Alternate Names:

CXCL12 chemokine (C-X-C motif) ligand 12; CXCL12/SDF-1 alpha; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1