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Simple Plex Human CXCL11/I-TAC Cartridge

Catalog #: SPCKB-PS-000369 Datasheet
Simple Plex Human CXCL11/I-TAC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD16, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000369
SPCKA-PS-000369
SPCKC-PS-000369
Control Products Available
898412: Simple Plex Control for Human CXCL11/I-TAC
Human I-TAC Simple Plex Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CXCL11/I-TAC Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.181 pg/mL
Assay Range
0.5 - 2,080 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human I-TAC.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human I-TAC in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 8 5
Mean (pg/mL) 22 1212 21.9 1164
Standard Deviation 0.809 83.9 1.31 173
CV% 3.7 6.9 6 14.9

Recovery

Recovery was not necessary as I-TAC shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human I-TAC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human I-TAC Ella Assay Linearity

Scientific Data

N/A Human I-TAC Simple Plex Assay Standard Curve View Larger

Human I-TAC Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Entrez Gene IDs:
6373 (Human); 56066 (Mouse)
Alternate Names:
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

FAQs

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