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Simple Plex Human CX3CL1/Fractalkine Cartridge

Catalog #: SPCKB-PS-000488 Datasheet
Simple Plex Human CX3CL1/Fractalkine assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000488
SPCKA-PS-000488
SPCKC-PS-000488
Control Products Available
898143: Simple Plex Control for Human CX3CL1/Fractalkine
Human Fractalkine Ella Assay
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CX3CL1/Fractalkine Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
18.2 pg/mL
Assay Range
56.8 - 86,710 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Specificity
Natural and recombinant human Fractalkine.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Fractalkine in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 110 102-127
EDTA Plasma (n=4) 101 93-112
Heparin Plasma (n=4) 99 93-113
Serum (n=4) 112 103-121
Urine (n=6) 92 77-103

Linearity

Samples containing and/or spiked with high concentrations of human Fractalkine were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Fractalkine Ella Assay Linearity

Scientific Data

N/A Human Fractalkine Simple Plex Assay Standard Curve View Larger

Human Fractalkine Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Entrez Gene IDs:
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names:
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

FAQs

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