Simple Plex Human CCL18/PARC Cartridge

Catalog #: SPCKB-PS-001408 Datasheet

Simple Plex Human CCL18/PARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001408
SPCKA-PS-001408
SPCKC-PS-001408

Control Products Available

898577: Simple Plex Control for Human CCL18/PARC

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CCL18/PARC Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL)

Sensitivity

0.06 pg/mL

Assay Range

0.1 - 1,120 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human CCL18.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CCL18 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	41
Mean (pg/mL)	7.63	378	7.07	367
Standard Deviation	0.552	23	0.497	41.6
CV%	7.2	6.1	7	11.3

Recovery

Recovery was not necessary as CCL18 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human PARC Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Entrez Gene IDs:

6362 (Human)

Alternate Names:

Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Related Research Areas

FAQs

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