Simple Plex Human CA125/MUC16 Cartridge
Simple Plex Human CA125/MUC16 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 18 |
| Mean (mU/mL) | 2954 | 141869 | 2885 | 130993 |
| Standard Deviation | 55.9 | 3239 | 152 | 8524 |
| CV% | 1.9 | 2.3 | 5.3 | 6.5 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 101 | 97-106 |
| Heparin Plasma (n=4) | 93 | 90-98 |
| Serum (n=4) | 95 | 88-99 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CA125/MUC16
The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.
FAQs
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